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The combination of sensitive microbore column (1.0 and 2.1 mm internal diameter) HPLC technology and microsequence analysis has enabled the positive identification of 125 of the total 179 amino acid residues (70%) in the molecule. The amino acid sequence data reported here permitted the isolation of a partial cDNA clone encoding LIF [Gearing et al. (1987) <jats:italic>EMBO J. 6<\/jats:italic>, 3995\u201340021. A candidate C\u2010terminus of the LIF molecule predicted from the amino acid sequence was confirmed by subsequent isolation of a cDNA clone corresponding to the C\u2010terminus of the protein.<\/jats:p><jats:p>No strong similarity was revealed when the amino acid sequence of LIF was compared with other haemopoietic growth factors, in particular granulocyte\u2010macrophage colony\u2010stimulating factor, granulocyte colony\u2010stimulating factor and tumour necrosis factor\u2010\u03b1 or interleukins. The protein sequence data reported here indicate three sites of post\u2010translational modification (N\u2010linked glycosylation).<\/jats:p>","DOI":"10.1111\/j.1432-1033.1988.tb14226.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T02:21:29Z","timestamp":1109902889000},"page":"541-547","source":"Crossref","is-referenced-by-count":28,"title":["Structural characterization of a murine myeloid leukaemia inhibitory factor"],"prefix":"10.1111","volume":"175","author":[{"given":"Richard J.","family":"SIMPSON","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas J.","family":"HILTON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edouard C.","family":"NICE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"RUBIRA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"METCALF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"GEARING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas M.","family":"GOUGH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicos A.","family":"NICOLA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_3_2_2","first-page":"389","volume":"230","author":"Metcalf D.","year":"1987","journal-title":"Proc. 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