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The positive clone contained a DNA fragment harbouring an open reading frame of 1536 nucleotides coding for a 512\u2010amino\u2010acid polypeptide with a calculated <jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> of 56 500. The deduced amino acid sequence reveals significant similarity to the sequence of the <jats:italic>Saccharomycopsis fibuligera<\/jats:italic> and <jats:italic>Aspergillus oryzae<\/jats:italic>\u03b1\u2010amylases. The <jats:italic>AMY1<\/jats:italic> gene was found to be expressed from its original promoter in <jats:italic>S. cerevisiae, Kluyveromyces lactis<\/jats:italic> and <jats:italic>Schizosaccharomyces pombe<\/jats:italic> leading to an active secreted gene product and thus enabling the different yeast transformants to grow on starch as a sole carbon source.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1989.tb15069.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T03:26:54Z","timestamp":1109906814000},"page":"699-706","source":"Crossref","is-referenced-by-count":37,"title":["Analysis of the \u03b1\u2010amylase gene of <i>Schwanniomyces occidentalis<\/i> and the secretion of its gene product in transformants of different yeast genera"],"prefix":"10.1111","volume":"184","author":[{"given":"Alexander W. 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