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We now report the isolation of the nuclear gene encoding this single\u2010subunit enzyme. Null mutants have been constructed by means of one\u2010step gene disruption. Oxygen\u2010uptake experiments, performed with mitochondria isolated from the mutant cells, showed that this NADH dehydrogenase catalyzes the oxidation of NADH generated inside the mitochondrion. Inactivation of this NADH dehydrogenase does not affect growth on glucose and ethanol, but growth on lactate, pyruvate and acetate is impaired or absent. This phenotype is discussed in terms of the interplay between different metabolic pathways in yeast.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1991.tb15775.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T14:15:40Z","timestamp":1109945740000},"page":"857-862","source":"Crossref","is-referenced-by-count":105,"title":["Isolation and inactivation of the nuclear gene encoding the rotenone\u2010insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1111","volume":"195","author":[{"given":"C. A. M.","family":"MARRES","sequence":"first","affiliation":[]},{"given":"S.","family":"de VRIES","sequence":"additional","affiliation":[]},{"given":"L. 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