{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:50:23Z","timestamp":1761058223256},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":4171,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1993,10]]},"abstract":"<jats:p>We have investigated the expression of nuclear\u2010encoded chloroplast proteins that are not associated with chlorophyll (the lumenal 33\u2010kDa and 23\u2010kDa polypeptides of the oxygen\u2010evolving system of photosystem II, plastocyanin and the Rieske Fe\/S protein) by comparing mRNA\u2010accumulation rates with those of the corresponding proteins during illumination of etiolated tobacco seedlings. Using subcellular fractionation, pulse\/chase, Northern and Western techniques, we found that the biogenesis and stability of these proteins are regulated both translationally, as well as post\u2010translationally, including the efficiency of mRNA uptake into polysomes, processes that operate between translation and assembly or monitor the status (soluble and membrane\u2010attached) of a terminally processed polypeptide. Polypeptide synthesis is generally not limited by mRNA amounts. For instance, steady\u2010state transcript levels may increase 10\u2010fold during illumination, while those associated with polysomes increase only 2\u20133\u2010fold without measurable influence on the rate of protein synthesis. The 23\u2010kDa and Rieske polypeptides are predominantely membrane associated, but plastocyanin and the 33\u2010kDa polypeptide are distributed among both soluble and membrane\u2010associated protein fractions. Plastocyanin appears to be comparably stable in both forms. However, for the 33\u2010kDa polypeptide, only the membrane\u2010attached form is stable (&gt;8h) and only this pool increases upon illumination. Its soluble form is rapidly degraded with a half\u2010life of approximately 1 h under the chosen conditions. Our findings probably reflect part of a more general regulatory principle operating in the differentiation and maintenance of subcellular structure.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1993.tb18252.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T09:20:31Z","timestamp":1109928031000},"page":"345-352","source":"Crossref","is-referenced-by-count":27,"title":["Post\u2010transcriptional and post\u2010translational regulatory steps are crucial in controlling the appearance and stability of thylakoid polypeptides during the transition of etiolated tobacco seedlings to white light"],"prefix":"10.1111","volume":"217","author":[{"given":"Rosario","family":"PALOMARES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhold G.","family":"HERRMANN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"OELM\u00dcLLER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"447","volume":"19","author":"Andersson B.","year":"1986","journal-title":"Encycl. Plant Physiol."},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb13101.x"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1981.tb05486.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240250102"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.3.2.203"},{"key":"e_1_2_2_7_1","author":"Bolle C.","year":"1993","journal-title":"Proc. Photosynth. Res."},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.34.060183.001325"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90290-X"},{"key":"e_1_2_2_10_1","doi-asserted-by":"crossref","first-page":"9641","DOI":"10.1016\/S0021-9258(18)47982-3","volume":"262","author":"Deng X. W.","year":"1987","journal-title":"J. Biol. Chem."},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77385-7"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.2.321"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1993.04010009.x"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.1.3.301"},{"key":"e_1_2_2_16_1","first-page":"285","volume-title":"Photosynthesis","author":"Gamble P. E.","year":"1989"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.2.5.369"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02668635"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3304-7_40"},{"key":"e_1_2_2_20_1","first-page":"233","volume-title":"Molecular form and function of the plant genome","author":"Herrmann R. G.","year":"1985"},{"key":"e_1_2_2_21_1","first-page":"275","volume-title":"Plant gene research","author":"Herrmann R. G.","year":"1992"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00023442"},{"key":"e_1_2_2_23_1","unstructured":"Jansen T.(1991)Ph. D. Thesis University of Munich."},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67359-4"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.88.4.1246"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09908.x"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(89)80086-6"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.39.060188.002355"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02402.x"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.11.4038"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1962.tb08052.x"},{"key":"e_1_2_2_34_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.92.2.434"},{"key":"e_1_2_2_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392323"},{"key":"e_1_2_2_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00446376"},{"key":"e_1_2_2_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392267"},{"key":"e_1_2_2_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(91)80257-I"},{"key":"e_1_2_2_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00196958"},{"key":"e_1_2_2_40_1","first-page":"4557","volume":"7","author":"Pandey N. B.","year":"1987","journal-title":"Cell. Biol."},{"key":"e_1_2_2_41_1","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_2_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90587-2"},{"key":"e_1_2_2_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.9.2632"},{"key":"e_1_2_2_44_1","first-page":"101","volume":"183","author":"Schrubar H.","year":"1990","journal-title":"Planta (Heidelb.)"},{"key":"e_1_2_2_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02464904"},{"key":"e_1_2_2_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00959531"},{"key":"e_1_2_2_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90341-7"},{"key":"e_1_2_2_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05692.x"},{"key":"e_1_2_2_49_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.3.3.285"},{"key":"e_1_2_2_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90600-3"},{"key":"e_1_2_2_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1987.tb11165.x"},{"key":"e_1_2_2_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.42.060191.002231"},{"key":"e_1_2_2_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425507"},{"key":"e_1_2_2_54_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.36.060185.003033"},{"key":"e_1_2_2_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00027317"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1993.tb18252.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1993.tb18252.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T04:56:55Z","timestamp":1698728215000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1993.tb18252.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,10]]}},"alternative-id":["10.1111\/j.1432-1033.1993.tb18252.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1993.tb18252.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10]]}}}