{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:15:34Z","timestamp":1779340534884,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":4079,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>We have isolated the basement membrane component nicein and performed rotary\u2010shadow analyses using electron microscopy that showed the presence of two forms (I and II) of the protein. Molecular cloning of the cDNA that codes for the 100\u2010kDa chain of the protein revealed that the sequence matches those independently identified for the 105\u2013155\u2010kDa subunit of kalinin, a recently identified basement membrane component. These data demonstrate that nicein and kalinin contain an identical chain. The length of the open reading frame in the cDNA (\u223c5200 nucleotides) and amino acid sequences obtained from the N\u2010terminus of the 105\u2010kDa kalinin chain showed the occurrence of a precursor polypeptide. This immature polypeptide is probably related to form I, observed by rotary shadowing, while the mature form is related to form II. It is noteworthy that nicein\/kalinin subunits share\u2010discrete sequence similarities with the B2 chain of human laminin, but with a cleavage occurring within domain III that eliminates domains IV and V from the final product. The sequence of this subunit is nearly identical to that of B2t, a recently described polypeptide supposed to be related to a new laminin variant. Since nicein\/kalinin expression is specifically impaired in the severe genodermatosis Herlitz junctional epidermolysis bullosa, the role and structure of this tissue\u2010restricted laminin variant is crucial for the understanding of epidermal\u2010dermal adhesion.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1994.tb19932.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T09:49:18Z","timestamp":1109929758000},"page":"209-218","source":"Crossref","is-referenced-by-count":88,"title":["The 100\u2010kDa chain of nicein\/kalinin is a laminin B2 chain variant"],"prefix":"10.1111","volume":"219","author":[{"given":"Jo\u00eblle","family":"VAILLY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"VERRANDO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010France","family":"CHAMPLIAUD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"GERECKE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Wolf","family":"WAGMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"BAUDOIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"ABERDAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"BURGESON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"BAUER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"ORTONNE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.6.2180767"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90121-2"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(88)90273-8"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.3.567"},{"key":"e_1_2_2_6_2","first-page":"295","volume":"69","author":"Marinkovitch M. P.","year":"1993","journal-title":"Lab. Invest."},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90972-4"},{"key":"e_1_2_2_8_2","first-page":"85","volume":"64","author":"Verrando P.","year":"1991","journal-title":"Lab. Invest."},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1992.tb00080.x"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12283807"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.06.110190.002333"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.679"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(75)80001-8"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12546943"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111297"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90568-9"},{"key":"e_1_2_2_17_2","author":"Verrando P.","year":"1993","journal-title":"J. Invest Dermatol."},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43211-5"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/300533a0"},{"key":"e_1_2_2_20_2","doi-asserted-by":"crossref","first-page":"9042","DOI":"10.1016\/S0021-9258(19)84485-X","volume":"261","author":"Lunstrum G. P.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37127-3"},{"key":"e_1_2_2_22_2","doi-asserted-by":"crossref","first-page":"5638","DOI":"10.1016\/S0021-9258(19)57262-3","volume":"261","author":"Morris N. P.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.5.1194"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.8998"},{"key":"e_1_2_2_25_2","first-page":"355","volume":"6","author":"Dessen P.","year":"1990","journal-title":"Comp. Appl. Biosci."},{"key":"e_1_2_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.20.6970"},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61070-1"},{"key":"e_1_2_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1408"},{"key":"e_1_2_2_30_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.695"},{"key":"e_1_2_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16358.x"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05836.x"},{"key":"e_1_2_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90092-D"},{"key":"e_1_2_2_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116033"},{"key":"e_1_2_2_35_2","doi-asserted-by":"publisher","DOI":"10.1159\/000132610"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1994.tb19932.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1994.tb19932.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T07:54:20Z","timestamp":1693468460000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1994.tb19932.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":34,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1111\/j.1432-1033.1994.tb19932.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1994.tb19932.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}