{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:48:51Z","timestamp":1773658131277,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T00:00:00Z","timestamp":1090540800000},"content-version":"vor","delay-in-days":2791,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:p>Molecular mechanisms involved in the nucleolytic cleavage at the 18S rRNA\/internal transcribed spacer 1 (ITS 1) junction, a late step of small\u2010subunit pre\u2010rRNA processing in vertebrates, remain largely unknown, mostly due to the lack of faithful <jats:italic>in vitro<\/jats:italic> assays. To identify the minimal <jats:italic>cis<\/jats:italic>\u2010acting signals required for this reaction, we studied the processing of truncated human rRNA gene transcripts transiently expressed upon transfection of rRNA minigenes into cultured mouse cells. We observed that processing at this site was faithfully reproduced with transcripts containing only 60 nucleotides of 18S rRNA and the adjacent 103 nucleotides of ITS 1, but was abolished or severely altered by further shortening of either sequence. Remarkably, this minimal transcript contains, within its 18S rRNA part, long sequences complementary to both U20 and U13 small nucleolar RNAs (snoRNAs). The <jats:italic>cis<\/jats:italic>\u2010acting elements essential for the reaction were studied further by site\u2010directed mutagenesis. The U20 snoRNA complementary region in 18S rRNA was not required for faithful processing at the 18S rRNA\/ITS 1 junction. Also, processing at this site was not appreciably altered by random substitution of proximal ITS 1 sequences (including the 5\u2032 terminal nucleotide) or of the terminal nucleotide of mature 18S rRNA. Substitutions in the four\u2010nucleotide loop of the 18S rRNA 3\u2032\u2010terminal stem\u2010loop, including the two adenosine residues substrates of dimethylation, did not alter appreciably the formation of the 18S rRNA 3\u2032 end, showing that the (methyl)<jats:sub>2<\/jats:sub>A1850 \u00b7 (methyl)<jats:sub>2<\/jats:sub>A1851 doublet was not required for processing at this site. Two highly conserved 18S rRNA elements acted as major <jats:italic>cis<\/jats:italic>\u2010acting signals for processing at the 3\u2032 end, the CAUU sequence immediately preceding the 3\u2032\u2010terminal nucleotide and the 3\u2032 strand of the 3\u2032\u2010terminal 18S rRNA helix, complementary to U13 snoRNA. Compensatory mutations, restoring the potential for helix formation, but not U13 snoRNA complementarity, did not restitute the cleavage at the 3\u2032 end of 18S rRNA. This suggests that U13 snoRNA may be a <jats:italic>trans<\/jats:italic>\u2010acting factor in the nucleolytic cleavage at the 3\u2032 end of 18S rRNA.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1996.0206r.x","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T09:08:12Z","timestamp":1090573692000},"page":"206-213","source":"Crossref","is-referenced-by-count":28,"title":["Processing of Mammalian rRNA Precursors at the 3\u2032 End of 18S rRNA"],"prefix":"10.1111","volume":"242","author":[{"given":"J\u00e9r\u00f4me","family":"Cavaill\u00e9","sequence":"first","affiliation":[]},{"given":"Asen A.","family":"Hadjiolov","sequence":"additional","affiliation":[]},{"given":"Jean\u2010Pierre","family":"Bachellerie","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,7,23]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)60051-3"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-8742-5"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)60629-7"},{"key":"e_1_2_2_5_2","first-page":"469","volume-title":"Ribosomal RNA: structure, evolution, gene expression and function in protein synthesis","author":"Sollner\u2010Webb B.","year":"1996"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.53.2.256-271.1989"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.3.629"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.11.1.458"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.8.2891"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.10.5990"},{"key":"e_1_2_2_11_2","first-page":"71","article-title":"The pathway to maturity: processing of ribosomal RNA in Saccharomyces cerevisiae","volume":"5","author":"Rau\u00e9 H. 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