{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T19:04:34Z","timestamp":1768417474176,"version":"3.49.0"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T00:00:00Z","timestamp":1172620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The dissimilatory reduction of sulfate contributes to the retention of sulfur in acidic mineratrophic peatlands. Novel sulfate\u2010reducing prokaryotes (SRPs) colonize these low\u2010sulfate fens. This study assessed the community structures of SRPs in a depth gradient (0\u201350\u2003cm) in a fen, located in the Fichtelgebirge (Spruce Mountains), Germany. Detection of SRPs with multiplex (terminal\u2010) restriction fragment length polymorphism analysis of amplified dissimilatory (bi)sulfite reductase genes (\n                    <jats:italic>dsrAB<\/jats:italic>\n                    ) separated three subgroups derived from (i) the upper 5 and 10\u2003cm, (ii) 15\u201325\u2003cm, and (iii) 30\u201350\u2003cm depth. Biogeochemical parameters measured in the soil solution from July 2001 to July 2004 documented that the upper 5\u201310\u2003cm were exposed to drying and oxygenation prior to sampling. Periodic oxygenation reached a maximum depth of 25\u2003cm in the water\u2010saturated fen and was concomitant with relative high concentrations of nitrate (120\u2003\u03bcM) and sulfate (up to 310\u2003\u03bcM). The fen soil was permanently anoxic below 30\u2003cm depth with average concentrations of sulfate below 40\u2003\u03bcM and maximum concentrations of methane. Cloning of\n                    <jats:italic>dsrAB<\/jats:italic>\n                    PCR products from 5, 20 and 40\u2003cm depth yielded a total of 84 unique\n                    <jats:italic>dsrAB<\/jats:italic>\n                    restriction patterns. Partial sequencing of 61 distinct clones resulted in 59 unique partial protein sequences that mainly clustered with DsrA sequences of uncultivated sulfate reducers.\n                    <jats:italic>Syntrophobacter fumaroxidans<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>Syntrophobacter wolinii<\/jats:italic>\n                    \u2010related bacteria appeared to be present only in 40\u2003cm depth. Differences in the SRP community structures suggested that SRPs present in the upper fen soil have to tolerate O\n                    <jats:sub>2<\/jats:sub>\n                    and even drying, whereas SRPs present in deep anoxic zones may act as syntrophic fermentors in cooperation with H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010utilizing methanogens.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2007.01251.x","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T04:56:11Z","timestamp":1172638571000},"page":"1317-1328","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["High unique diversity of sulfate\u2010reducing prokaryotes characterized in a depth gradient in an acidic fen"],"prefix":"10.1111","volume":"9","author":[{"given":"Achim","family":"Schmalenberger","sequence":"first","affiliation":[]},{"given":"Harold 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