{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T13:55:49Z","timestamp":1771682149298,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T00:00:00Z","timestamp":1267142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pectobacterium carotovorum<\/jats:italic>\n                    SCRI193 is a phytopathogenic Gram\u2010negative bacterium. In this study, we have identified a novel cryptic pigment biosynthetic locus in\n                    <jats:italic>P. carotovorum<\/jats:italic>\n                    SCRI193 which we have called the\n                    <jats:italic>Pectobacterium<\/jats:italic>\n                    orange pigment (\n                    <jats:italic>pop<\/jats:italic>\n                    ) cluster. The\n                    <jats:italic>pop<\/jats:italic>\n                    cluster is flanked by two tRNA genes and contains genes that encode non\u2010ribosomal peptide synthases and polyketide synthase and produces a negatively charged polar orange pigment. Orange pigment production is activated when an adjacent transcriptional activator sharing sequence similarity with the\n                    <jats:italic>Erwinia<\/jats:italic>\n                    virulence regulator (Evr) is overexpressed. Evr was shown to positively activate its own transcription and that of the pigment biosynthetic genes and an unlinked locus encoding a phenomycin homologue. In addition, the expression of Evr and orange pigment production was shown to be regulated by\n                    <jats:italic>N\u2010<\/jats:italic>\n                    (3\u2010oxohexanoyl)\u2010HSL (OHHL) quorum sensing and have a virulence phenotype in potato. Finally, by comparative genomics and Southern blotting we demonstrate that this pigment biosynthetic cluster is present in multiple\n                    <jats:italic>P. carotovorum<\/jats:italic>\n                    spp.,\n                    <jats:italic>Pectobacterium brasiliensis<\/jats:italic>\n                    1692 and a truncated version of the cluster is present in\n                    <jats:italic>Pectobacterium atrosepticum<\/jats:italic>\n                    . The conserved nature of this cluster in\n                    <jats:italic>P. carotovorum<\/jats:italic>\n                    and\n                    <jats:italic>P. brasiliensis<\/jats:italic>\n                    suggests that the\n                    <jats:italic>pop<\/jats:italic>\n                    cluster has an important function in these broad\u2010host\u2010range soft rotting bacteria, which is no longer required in the narrow\u2010host\u2010range\n                    <jats:italic>P. atrosepticum<\/jats:italic>\n                    SCRI1043.\n                  <\/jats:p>","DOI":"10.1111\/j.1462-2920.2010.02170.x","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T02:28:34Z","timestamp":1267151314000},"page":"1811-1827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Quorum sensing\u2010controlled Evr regulates a conserved cryptic pigment biosynthetic cluster and a novel phenomycin\u2010like locus in the plant pathogen,\n                    <i>Pectobacterium carotovorum<\/i>"],"prefix":"10.1111","volume":"12","author":[{"given":"Neil R.","family":"Williamson","sequence":"first","affiliation":[]},{"given":"Paul M. 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