{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:00:08Z","timestamp":1777438808296,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,9,22]],"date-time":"2004-09-22T00:00:00Z","timestamp":1095811200000},"content-version":"vor","delay-in-days":2275,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>We examined the relationship between calcineurin (protein phosphatase 2B (PP2B)) and voltage\u2010operated Ca<jats:sup>2+<\/jats:sup> channels (VOCCs) in NG108\u201315 cells. PP2B expression in NG108\u201315 cells was altered by transfection with plasmid constructs containing a full length cDNA of human PP2B\u03b2<jats:sub>3<\/jats:sub> in sense (CN\u201015) and antisense (CN\u201021) orientation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Confocal immunocytochemical localization showed that in wild\u2010type cells, PP2B immunoreactivity is uniformly distributed in undifferentiated cells and located at the inner surface of soma membrane and neurites in differentiated cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>To test the Ca<jats:sup>2+<\/jats:sup> dependence of the VOCC, we used high\u2010frequency stimulation (HFS). The L\u2010 and N\u2010type VOCCs decreased by 37 and 52 %, respectively, whereas the T\u2010type current was only marginally sensitive to this procedure. FK\u2010506 (2 \u03bcM), a specific blocker of PP2B, reduced the inhibition of L\u2010 and N\u2010type VOCCs induced by HFS by 30 and 33 %, respectively.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In CN\u201015\u2010transfected cells overexpressing PP2B, total high\u2010voltage\u2010activated (HVA) VOCCs were suppressed by about 60 % at a test potential of +20 mV. Intracellular addition of EGTA or FK\u2010506 into CN\u201015\u2010transfected cells induced an up to 5\u2010fold increase of HVA VOCCs.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings indicate that PP2B activity does not influence the expression of HVA Ca<jats:sup>2+<\/jats:sup> channels, but modulates their function by Ca<jats:sup>2+<\/jats:sup>\u2010dependent dephosphorylation. Thus HVA VOCCs, in a phosphorylated state under control conditions, are downregulated by PP2B upon stimulation, with the major effect being on N\u2010type VOCCs.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.1998.371bk.x","type":"journal-article","created":{"date-parts":[[2004,9,22]],"date-time":"2004-09-22T09:15:59Z","timestamp":1095844559000},"page":"371-385","source":"Crossref","is-referenced-by-count":28,"title":["Calcineurin involvement in the regulation of high\u2010threshold Ca<sup>2+<\/sup> channels in NG108\u201315 (rodent neuroblastoma \u00d7 glioma hybrid) cells"],"prefix":"10.1113","volume":"510","author":[{"given":"E. A.","family":"Lukyanetz","sequence":"first","affiliation":[]},{"given":"T. P.","family":"Piper","sequence":"additional","affiliation":[]},{"given":"T. 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