{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T01:52:04Z","timestamp":1775353924749,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,9,22]],"date-time":"2004-09-22T00:00:00Z","timestamp":1095811200000},"content-version":"vor","delay-in-days":2305,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Heterologous expression studies of the \u03b15 subunit of the neuronal acetylcholine receptor (nAChR) gene family have demonstrated that it can participate in the function of ACh\u2010gated channels if co\u2010expressed with another \u03b1\u2010 and a \u03b2\u2010subunit. Previous studies also indicate prominent expression of \u03b15 in both central and peripheral nervous systems. The participation of \u03b15 in native nAChRs and its functional role in these channels is, however, unknown.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In this study, we present evidence that \u03b15 has a role in at least two distinct subtypes of nAChR complexes expressed by embryonic chick sympathetic neurones.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>\u03b15 contributes not only to agonist but also to antagonist sensitivity of natively expressed nAChR channels. Functional deletion of the \u03b15 subunit by antisense oligonucleotide treatment removes the nAChRs with relatively low affinity to ACh and cytisine. Deletion of \u03b15 also eliminates channels that are blocked by the \u03b17\u2010specific antagonist methyllycaconitine (MLA) while increasing the percentage of current carried by nAChRs that are sensitive to \u03b1\u2010bungarotoxin (\u03b1\u2010BgTx).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Single channel analyses indicate that functional deletion of \u03b15 results in the deletion of both the \u2018brief\u2019 and \u2018long\u2019 open duration, 50 pS subtypes of nAChR channels while increasing the expression of the 18 pS, \u03b1\u2010BgTx\u2010sensitive native nAChRs normally detected in sympathetic neurones at later developmental stages.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The biophysical and pharmacological profiles of native nAChRs revealed by this study and previous work are discussed in the context of a proposed model of the nAChR channels expressed by chick sympathetic neurones throughout development.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/j.1469-7793.1998.667bm.x","type":"journal-article","created":{"date-parts":[[2004,9,22]],"date-time":"2004-09-22T09:25:42Z","timestamp":1095845142000},"page":"667-681","source":"Crossref","is-referenced-by-count":74,"title":["Functional contribution of the \u03b15 subunit to neuronal nicotinic channels expressed by chick sympathetic ganglion neurones"],"prefix":"10.1113","volume":"509","author":[{"given":"C. Ron","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorna W.","family":"Role","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,22]]},"reference":[{"key":"e_1_2_6_1_1","first-page":"1455","article-title":"Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. I. 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