{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:54:14Z","timestamp":1759938854933},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,12]],"date-time":"2006-12-12T00:00:00Z","timestamp":1165881600000},"content-version":"vor","delay-in-days":10391,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1978,7]]},"abstract":"<jats:p><jats:bold>Abstract\u2014<\/jats:bold> Radioimmunoassays (RIAs) selective for methionine\u2010enkephalin (Met\u2010ENK) and leucineenkephalin (Leu\u2010ENK) have been developed using competition towards binding of 10 pM <jats:sup>125<\/jats:sup>I\u2010enkephalins to antibodies raised in rabbits against ENKs coupled to ovalbumin with carbodiimide. The high sensitivity of the RIAs (IC<jats:sub>50<\/jats:sub> 0.57 n<jats:sc>m<\/jats:sc> and 0.55 n<jats:sc>m<\/jats:sc> for Met\u2010 and Leu\u2010ENK, respectively) allowed estimation of the enkephalin content in extracts of all rat brain regions. Regional levels are compared with those determined on the same extracts by a radioreceptor assay (RRA) using competition towards binding of 5 n<jats:sc>m<\/jats:sc> [<jats:sup>3<\/jats:sup>H]Leu\u2010ENK to rat striatal membranes. Optimal conditions for killing the animals and extracting the endorphins have been carefully investigated: killing by rapid microwave irradiation was not found necessary as long as brain regions were homogenized into 0.1 <jats:sc>n<\/jats:sc>\u2010HCl before deproteinization.<\/jats:p><jats:p>Marked differences both in total endorphins (RRA) and ENKs (RIA) between regions are observed with similar ranking of the various regions: highest levels are found in striatum and hypothalamus and lowest in cerebellum and hippocampus. In each region the total ENK levels (RIA) represent only 2\u201313% of the total endorphins (RRA) suggesting the presence of large amounts of endorphins other than the ENKs.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1978.tb12429.x","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T13:02:15Z","timestamp":1166014935000},"page":"29-39","source":"Crossref","is-referenced-by-count":137,"title":["RADIOIMMUNOASSAY OF METHIONINE\u2010AND LEUCINE\u2010ENKEPHALINS IN REGIONS OF RAT BRAIN AND COMPARISON WITH ENDORPHINS ESTIMATED BY A RADIORECEPTOR ASSAY"],"prefix":"10.1111","volume":"31","author":[{"given":"C.","family":"Gros","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pradelles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Rouget","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Bepoldin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Dray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Fournie\u2010Zaluski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. P.","family":"Roques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Pollard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Llorens\u2010Cortes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Schwartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,12]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"73","volume":"284","author":"Audigier Y.","year":"1977","journal-title":"C.r. hebd. S\u00e9ant: Acad. Sci., Paris"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(77)90220-5"},{"key":"e_1_2_2_4_1","first-page":"63","volume-title":"Opiate and Endogenous Opioid Peptides","author":"Akil H.","year":"1976"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/260793a0"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(76)90291-5"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(77)90504-5"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(75)90272-6"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(76)90857-3"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)60228-5"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(76)90063-4"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(76)90823-8"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(77)80128-2"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1966.tb09873.x"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.108.1.37"},{"key":"e_1_2_2_16_1","first-page":"783","volume":"232","author":"Guillemin R.","year":"1976","journal-title":"C.r. hebd. S\u00e9anc. Acad. Sci. Paris"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.7.3081"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(75)90391-1"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/258577a0"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0910043"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/268549a0"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90296-1"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(77)90147-9"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/267495a0"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1130299"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)90619-2"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)90585-X"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlcr.2590060308"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(75)90270-2"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.8.2243"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/268745a0"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/262778a0"},{"key":"e_1_2_2_34_1","first-page":"35","volume-title":"Opiate and Endogenous Opioid Peptides","author":"Ross M.","year":"1976"},{"key":"e_1_2_2_35_1","first-page":"245","volume-title":"Advances in Biochemical Psychopharmacology","author":"Schwartz J. C.","year":"1978"},{"key":"e_1_2_2_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.7.2515"},{"key":"e_1_2_2_37_1","first-page":"41","volume-title":"Opiate and Endogenous Opioid Peptides","author":"Simantov R.","year":"1976"},{"key":"e_1_2_2_38_1","first-page":"57","volume-title":"Opiate and Endogenous Opioid Peptides","author":"Smith T. W.","year":"1976"},{"issue":"1","key":"e_1_2_2_39_1","first-page":"55","volume":"35","author":"Terenius L.","year":"1974","journal-title":"Acta Pharmac. tox."},{"key":"e_1_2_2_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(75)90271-4"},{"key":"e_1_2_2_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(76)80767-3"},{"key":"e_1_2_2_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(77)90112-5"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.1978.tb12429.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.1978.tb12429.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T20:52:56Z","timestamp":1699131176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.1978.tb12429.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,7]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,7]]}},"alternative-id":["10.1111\/j.1471-4159.1978.tb12429.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.1978.tb12429.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,7]]}}}