{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T16:02:46Z","timestamp":1698249766632},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":7704,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1985,9]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> An enzymatic microassay method for glutamate decarboxylase (GAD) and \u03b3\u2010aminobutyric acid (GABA) was improved to a degree yielding high sensitivity and low blank. Single cell bodies of anterior horn cells and dorsal root ganglion cells were dissected out from the freeze\u2010dried sections of rabbit and chicken spinal cords and Purkinje cell bodies from those of rabbit cerebellum. A minute amount of GABA, present in single neurons or synthesized by GAD in single neurons, was enzymatically converted to NADPH. The NADPH was amplified 10,000\u2010350,000\u2010fold and measured, using an enzymatic amplification reaction (NADP cycling). GAD was contained in all Purkinje cell bodies and its average activity was four\u2010 to fivefold higher than those of the molecular and granular layers of rabbit cerebellum. The GABA concentration was threefold higher in Purkinje cell bodies than in these layers. GAD activity, at a level similar to that in the cerebellar layers, was found in almost all the cell bodies of anterior horn cells from rabbit and chicken. GABA was detected in 40% of rabbit neurons and not in chicken neurons. Dorsal root ganglion cells from both species contained no measurable GAD or GABA.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1985.tb04053.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T17:55:06Z","timestamp":1160070906000},"page":"732-738","source":"Crossref","is-referenced-by-count":10,"title":["Glutamate Decarboxylase Activities in Single Vertebrate Neurons"],"prefix":"10.1111","volume":"45","author":[{"given":"Yoshiya L.","family":"Murashima","sequence":"first","affiliation":[]},{"given":"Takahiko","family":"Kato","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1016\/S0021-9258(18)70204-4","article-title":"The distribution of glutamic decarboxylase in the nervous system of the rhesus monkey","volume":"234","author":"Albers R. W.","year":"1959","journal-title":"J. Biol. 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