{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T07:06:10Z","timestamp":1757574370610},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":7947,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:title>Abstract:<\/jats:title><jats:p>Specific binding of [<jats:sup>35<\/jats:sup>S]<jats:italic>t<\/jats:italic>\u2010butylbicyclophosphorothionate (TBPS) to rat brain membranes (RBM) is enhanced nine\u2010fold by EDTA\/water dialysis and 1.3\u2010 to 4.2\u2010fold by 50 n<jats:italic>M<\/jats:italic> ketosteroid R 5135, or 5 m<jats:italic>M<\/jats:italic> 3\u2010[(3\u2010cholamidopropyl)dimethylammonio]\u20101\u2010propanesulfonate (CHAPS) or related piperazine\u2010<jats:italic>N<\/jats:italic>\u2010alkanesulfonate buffers, or extensive washing with NaCl\/Na phosphate or Na phosphate\/citrate solution. About one\u2010fifth of the [<jats:sup>35<\/jats:sup>S]TBPS binding capacity appears in the soluble fraction whereas the rest remains in particulate form on treatment of the EDTA\/water\u2010dialyzed RBM with 20 m<jats:italic>M<\/jats:italic> CHAPS. Similar <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> values (64\u201386 n<jats:italic>M<\/jats:italic>) are obtained for the original EDTA\/water\u2010dialyzed membranes and the CHAPS\u2010treated and\/or \u2010solubilized preparations. The <jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub> of the EDTA\u2010treated RBM is reduced five\u2010fold on solubilization with CHAPS. The potency for displacement of [<jats:sup>35<\/jats:sup>S]TBPS changes in the presence of CHAPS or on CHAPS solubilization: \u03b3\u2010aminobutyric acid (GABA) and muscimol inhibit specific [<jats:sup>35<\/jats:sup>S]TBPS binding more strongly in the absence than in the presence of CHAPS; TBPS, picrotoxinin, and photoheptachlor epoxide are almost equally active with RBM, RBM + CHAPS, and RBM solubilized with CHAPS. Levels of (1<jats:italic>R,\u03b1S<\/jats:italic>)\u2010<jats:italic>cis<\/jats:italic>\u2010cypermethrin and dimethylbutylbarbiturate which are inhibitory with RBM are moderately stimulatory after TBPS receptor solubilization. Thus CHAPS defines three regions of the GABA receptor\u2010ionophore complex, i.e., the GABA and benzodiazepine receptors, the TBPS\/picrotoxinin\/polychlorocycloalkane receptor(s), and the sites at which the \u03b1\u2010cyano pyrethroid and the barbiturate interact with TBPS binding.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1985.tb07119.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T16:00:13Z","timestamp":1160064013000},"page":"110-116","source":"Crossref","is-referenced-by-count":31,"title":["Solubilization and Detergent Effects on Interactions of Some Drugs and Insecticides with the <i>t<\/i>\u2010Butylbicyclophosphorothionate Binding Site Within the \u03b3\u2010Aminobutyric Acid Receptor\u2010Ionophore Complex"],"prefix":"10.1111","volume":"44","author":[{"given":"Josef","family":"Seifert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E.","family":"Casida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"57","volume-title":"Neurotransmitter Receptor Binding","author":"Bennett J. 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