{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:58:14Z","timestamp":1761393494780},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":7643,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1985,11]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> Mn<jats:sup>2+<\/jats:sup> greatly increases the incorporation of <jats:italic>myo<\/jats:italic>\u2010[<jats:sup>3<\/jats:sup>H]inositol into phosphatidylinositol (PI) of brain and other tissues by stimulating the activity of a PI\u2010<jats:italic>myo<\/jats:italic>inositol exchange enzyme. This study examined the ability of norepinephrine (NE) and carbachol to stimulate the hydrolysis of [<jats:sup>3<\/jats:sup>H]PI formed in the absence and presence of Mn<jats:sup>2+<\/jats:sup>\u2010stimulated [<jats:sup>3<\/jats:sup>H]inositol exchange. Rat cerebral cortical slices were incubated with <jats:italic>myo<\/jats:italic>\u2010[<jats:sup>3<\/jats:sup>H]inositol for 60 min in an <jats:italic>N<\/jats:italic>\u20102\u2010hydroxyethyl piperazine\u2010<jats:italic>N\u2032<\/jats:italic>\u20102\u2010ethanesulfonic acid (HEPES) buffer without or with MnCl<jats:sub>2<\/jats:sub> (1 m<jats:italic>M<\/jats:italic>). The tissue was washed and further incubated with unlabeled <jats:italic>myo<\/jats:italic>\u2010inositol and LiCl (10 m<jats:italic>M<\/jats:italic>). Prelabeled slices were then incubated with NE (0.1 m<jats:italic>M<\/jats:italic>) or carbachol (1 m<jats:italic>M<\/jats:italic>) to induce agonist\u2010stimulated [<jats:sup>3<\/jats:sup>H]PI hydrolysis. Mn<jats:sup>2+<\/jats:sup> treatment resulted in eight\u2010 and sixfold increases in control levels of [<jats:sup>3<\/jats:sup>H]PI and [<jats:sup>3<\/jats:sup>H]inositol monophosphate ([<jats:sup>3<\/jats:sup>H]IP), respectively. Both NE and carbachol stimulated [<jats:sup>3<\/jats:sup>H]IP formation in tissue prelabeled without or with manganese. However, the degree of stimulation (percentage of control values) was greatly attenuated in the presence of Mn<jats:sup>2+<\/jats:sup>. In the absence of Mn<jats:sup>2+<\/jats:sup> treatment, NE decreased [<jats:sup>3<\/jats:sup>H]PI radioactivity in the tissue to 80% of control values. However, NE did not decrease [<jats:sup>3<\/jats:sup>H]PI radioactivity in the Mn<jats:sup>2+<\/jats:sup> \u2010treated tissue. These data demonstrate that Mn<jats:sup>2+<\/jats:sup> stimulates incorporation of <jats:italic>myo<\/jats:italic>\u2010[<jats:sup>3<\/jats:sup>H]inositol into a pool of PI in brain that has a rapid turnover but is not coupled to agonist\u2010induced hydrolysis.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1985.tb07216.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T18:41:32Z","timestamp":1160073692000},"page":"1481-1486","source":"Crossref","is-referenced-by-count":33,"title":["Manganese Stimulates the Incorporation of [<sup>3<\/sup>H]Inositol into a Pool of Phosphatidylinositol in Brain That Is Not Coupled to Agonist\u2010Induced Hydrolysis"],"prefix":"10.1111","volume":"45","author":[{"given":"Darryle D.","family":"Schoepp","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)77342-6"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)70226-3"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2120849"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb02798.x"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1950301"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(83)90092-4"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb06674.x"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(83)90146-7"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1800655"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(83)90093-0"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb12858.x"},{"key":"e_1_2_2_13_1","doi-asserted-by":"crossref","first-page":"10896","DOI":"10.1016\/S0021-9258(19)70391-3","article-title":"The effects of lithium ion and other agents on the activity of myo\u2010inositol 1\u2010phosphatase from bovine brain","volume":"255","author":"Hallcher L. M.","year":"1980","journal-title":"J. Biol. Chem."},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(71)90078-6"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(74)90197-0"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(57)03413-8"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/309795a0"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6147898"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)69403-7"},{"key":"e_1_2_2_20_1","first-page":"117","volume-title":"Remington's Pharmaceutical Sciences","author":"Randolph L. K.","year":"1975"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2190655"},{"key":"e_1_2_2_22_1","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/S0021-9258(19)45674-3","article-title":"Effects of acetylcholine on labeling of phosphatidate and phosphoinositides by [32P]orthophosphate in nerve ending fractions of guinea pig cortex","volume":"247","author":"Schacht J.","year":"1972","journal-title":"J. Biol. Chem."},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1984.tb06106.x"},{"key":"e_1_2_2_24_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1042\/bj1110147","article-title":"The deposition and metabolism of polyphosphoinositides in rat and guinea\u2010pig brain during development","volume":"111","author":"Sheltaway A.","year":"1969","journal-title":"Biochem. J."},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(80)90467-1"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90304-6"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1983.tb08073.x"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(79)81228-4"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.1985.tb07216.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.1985.tb07216.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T18:40:35Z","timestamp":1697827235000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.1985.tb07216.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1985,11]]}},"alternative-id":["10.1111\/j.1471-4159.1985.tb07216.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.1985.tb07216.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,11]]}}}