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Autoradiography revealed a high density of [<jats:sup>3<\/jats:sup>H]HC\u20103 binding sites in brain regions with a high density of cholinergic terminals, such as the interpeduncular nucleus, caudate\u2010putamen, and olfactory tubercle. This distribution of [<jats:sup>3<\/jats:sup>H]HC\u20103 binding sites was in close agreement with the amounts of choline acetyltrans\u2010ferase in specific nuclei and subregions of rat brain. Destruction of presynaptic cholinergic projections in the cerebral cortex and the basal ganglia by injection of excitotoxins reduced [<jats:sup>3<\/jats:sup>H]HC\u20103 binding by 40\u201350%. These data indicate that sodium\u2010dependent [<jats:sup>3<\/jats:sup>H]HC\u20103 binding sites are related to the choline transport system present in cholinergic neurons.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1986.tb12993.x","type":"journal-article","created":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T11:41:33Z","timestamp":1166010093000},"page":"483-488","source":"Crossref","is-referenced-by-count":54,"title":["Identification of Sodium\u2010Dependent, High\u2010Affinity Choline Uptake Sites in Rat Brain with [<sup>3<\/sup>H]Hemicholinium\u20103"],"prefix":"10.1111","volume":"46","author":[{"given":"Scott","family":"Manaker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline M.","family":"Wieczorek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas C.","family":"Rainbow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,12]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90806-4"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902160106"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(75)90174-5"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(85)90065-2"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.03-05-01069.1983"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1975.tb07624.x"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/263244a0"},{"key":"e_1_2_2_9_1","first-page":"427","volume-title":"Chemical Neuroanatomy","author":"Graybiel A. 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