{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T20:31:53Z","timestamp":1762979513504},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":7036,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1987,7]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> Rat glioma C6 cells, cultured in the presence of the tricyclic antidepressant desipramine, lost a significant number of \u03b2\u2010adrenergic receptors in a time\u2010and dose\u2010dependent manner. A similar loss was observed whether binding was determined on intact cells with the hydrophilic \u03b2\u2010adrenergic antagonist (\u00b1)\u2010[<jats:sup>3<\/jats:sup>H]4\u2010(3\u2010<jats:italic>tert<\/jats:italic>\u2010butylamino\u20102\u2010hydroxypropoxyl)benzimidazole \u20102 \u2010 on HCl ([<jats:sup>3<\/jats:sup>H]CGP\u201012177) or on cell lysates with the more hydrophobic antagonists [<jats:sup>125<\/jats:sup>I]iodocyanopindolol or [<jats:sup>3<\/jats:sup>H]dihydroalprenolol. When stimulated with the agonist isoproterenol, desipramine\u2010treated cells accumulated less cyclic AMP than control cells. The affinity of the \u03b2\u2010adrenergic receptors for either antagonist or agonist was unchanged after desipramine treatment. Desipramine interacted only weakly with the receptors and competed for [<jats:sup>125<\/jats:sup>I]iodocyanopindolol binding with a <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> of 30 \u03bc<jats:italic>M.<\/jats:italic> The presence in the culture medium of alprenolol or propranolol, potent \u03b2\u2010adrenergic antagonists, however, did not prevent the reduction in receptors by desipramine. Desipramine also caused a loss of \u03b2\u2010adrenergic receptors from cells maintained in serum\u2010free medium and the cells themselves did not contain or secrete endogenous catecholamines. Although desipramine is a potent inhibitor of catecholamine uptake, it appears unlikely that the observed loss of \u03b2\u2010adrenergic receptors in rat glioma C6 cells exposed to the drug is due to an increase in extracellular catecholamine levels or to a direct interaction with the receptors.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1987.tb03427.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T20:20:15Z","timestamp":1160079615000},"page":"282-289","source":"Crossref","is-referenced-by-count":40,"title":["Effect of the Tricyclic Antidepressant Desipramine on \u03b2\u2010Adrenergic Receptors in Cultured Rat Glioma C6 Cells"],"prefix":"10.1111","volume":"49","author":[{"given":"Peter H.","family":"Fishman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P. 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