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The ADP\u2010ribosylation in detergent solution retains the differential sensitivity to guanine nucleotide analogues. This reaction also removes the partial inhibition by the guanine nucleotides of the binding of opioid agonists, as does the same treatment in the membranes. The partial inhibition of agonist binding by Na<jats:sup>+<\/jats:sup>, however, is left unchanged. The binding of the antagonist naloxone is little affected by Na<jats:sup>+<\/jats:sup> or by guanine nucleotides in the treated membranes, but the treated soluble receptors show an enhanced binding in high\u2010Na<jats:sup>+<\/jats:sup> medium, although still guanine nucleotide insensitive. The data suggest that the toxin reaction in the absence of guanine nucleotides and agonist stabilizes the opioid receptor in a receptor\u2010G\u2010protein coupled state which is no longer sensitive to guanine nucleotides but retains its sensitivity to the Na<jats:sup>+<\/jats:sup> ions.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1988.tb04843.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T21:55:42Z","timestamp":1160085342000},"page":"114-121","source":"Crossref","is-referenced-by-count":10,"title":["ADP\u2010Ribosylation with Pertussis Toxin Modulates the GTP\u2010Sensitive Opioid Ligand Binding in Digitonin\u2010Soluble Extracts of Rat Brain Membranes"],"prefix":"10.1111","volume":"51","author":[{"given":"Yung H.","family":"Wong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine D.","family":"Demoliou\u2010Mason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric A.","family":"Barnard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(85)80185-6"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(78)90602-1"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00711077"},{"key":"e_1_2_2_5_1","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1016\/S0021-9258(18)33004-7","article-title":"Pertussis toxin inhibits enkephalin stimulation of GTPase of NG 108\u201315 cells","volume":"258","author":"Burns D. 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