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In the <jats:italic>\u03bc<\/jats:italic>\u2010preparation, but not in the <jats:italic>K<\/jats:italic>\u2010preparation, opioids inhibited the basal and the forskolin\u2010stimulated adenylate cyclase activity in a dose\u2010dependent manner and stereospecifically. The inhibition was in the 20\u201330% range, required the presence in the assay medium of Mg<jats:sup>2+<\/jats:sup> and of GTP, but was independent of the presence of Na<jats:sup>+<\/jats:sup>. Pharmacological characterization of the inhibitory response in the rabbit cerebellum clearly showed that it was under the control of a <jats:italic>\u03bc<\/jats:italic>\u2010opioid binding site, with the effect being elicited by nonselective (etorphine and morphine) and <jats:italic>\u03bc<\/jats:italic>\u2010selective (Tyr\u2010D\u2010Ala\u2010Gly\u2010Me\u2010Phe\u2010Gly\u2010ol) agonists, whereas \u03b4\u2010 and <jats:italic>K<\/jats:italic>\u2010selective agonists were almost totally ineffective. ADP ribosylation of inhibitory GTP\u2010binding protein by pertussis toxin failed to block the inhibitory effect of opioids, and data presented suggest that this failure is likely to be the consequence of a limited access of the toxin to its substrate in rabbit cerebellum membranes.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1990.tb01908.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T23:53:10Z","timestamp":1160092390000},"page":"562-570","source":"Crossref","is-referenced-by-count":25,"title":["\u03bc\u2010Opioid Receptors and Not <i>K<\/i>\u2010Opioid Receptors Are Coupled to the Adenylate Cyclase in the Cerebellum"],"prefix":"10.1111","volume":"54","author":[{"given":"J.","family":"Polastron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.\u2010J.","family":"Boyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Quertermont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010P.","family":"Thouvenot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010C.","family":"Meunier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ph.","family":"Jauzac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb09130.x"},{"key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0021-9258(17)31264-4","article-title":"K\u2010Opiate agonists inhibit Ca2+ influx in rat spinal cord dorsal root ganglion coculture.","volume":"264","author":"Attali B.","year":"1989","journal-title":"J. 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