{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:53:43Z","timestamp":1760853223485},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":5544,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A protein that binds vesamicol has been purified from a soluble fraction of the <jats:italic>Torpedo<\/jats:italic> electric organ homogenate that docs not contain synaptic vesicles. The purified vesamicol\u2010binding protein (VBP) has a molecular mass of 470 kDa composed of 30\u2010 and 24\u2010kDa subunits. Chemical deglycosylation yielded a single, heterogeneous protein of 24 kDa. The 30\u2010kDa subunit is also sensitive to endo\u2010\u03b2\u2010galactosidase. The dissociation constant of the VBP vesamicol complex is 0.9 <jats:italic>\u03bcM<\/jats:italic>, and the <jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub> is 5,500 pmol\/mg. Antiserum raised to the 30\u2010kDa subunit cross\u2010reacts with the 24\u2010kDa subunit, but not with synaptic vesicles. Drug binding studies and Western blot analysis show that VBP is present in other <jats:italic>Torpedo<\/jats:italic> tissues as well as mammalian brain. Immunofluorescence microscopy demonstrates that VBP\u2010like immunoreactivity is not localized exclusively to the nerve terminal regions of the electric organ. Thermal stability, the pH dependence of vesamicol binding, and pharmacological comparisons demonstrate that the VBP is not the cholinergic synaptic vesicle receptor for vesamicol. The implications of this finding for current efforts to develop in vivo diagnostics of cholinergic nerve terminal status based on vesamicol are discussed.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1991.tb03780.x","type":"journal-article","created":{"date-parts":[[2006,10,6]],"date-time":"2006-10-06T01:59:56Z","timestamp":1160099996000},"page":"509-519","source":"Crossref","is-referenced-by-count":29,"title":["Purification and Characterization of a Nonvesicular Vesamicol\u2010Binding Protein from Electric Organ and Demonstration of a Related Protein in Mammalian Brain"],"prefix":"10.1111","volume":"57","author":[{"given":"Barry W.","family":"Hicks","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary A.","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley M.","family":"Parsons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890020504"},{"key":"e_1_2_2_3_1","first-page":"48","article-title":"Pharmacological characterization of the acetylcholine transporter in purified Torpedo electric organ synaptic vesicles","volume":"24","author":"Anderson D. 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