{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,8]],"date-time":"2024-09-08T01:53:00Z","timestamp":1725760380285},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":5725,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1991,2]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> A cDNA probe specific to microtubule\u2010associated protein 2 (MAP2) was used to study the expression of the mRNAs encoding the high\u2010 and low\u2010molecular\u2010weight MAP2 variants in cultured neurons and astrocytes. The timing and relative abundance of these MAP2 transcripts and of their encoded proteins were also studied in the developing cerebral hemispheres and cerebellum of the mouse. A 9\u2010kb mRNA, known to encode high\u2010molecular\u2010weight MAP2, was expressed in cultured astrocytes, albeit at a lower level than in neurons. The 6\u2010kb transcript, recently shown to encode low\u2010molecular\u2010weight MAP2 (MAP2c), was expressed in neurons and was the predominant MAP2 transcript of the astrocytes. The level of the 9\u2010 and 6\u2010kb transcripts decreased at late stages of astroglial and neuronal cell culture. The 9\u2010kb mRNA was detected in the cerebellum and cerebral hemispheres at every developmental stage. Although the levels of this mRNA varied slightly in the cerebral hemispheres, its expression was biphasic in the cerebellum. This might be explained by the differences in timing of development of the various neuronal cell types formed in these two brain areas. The 6\u2010kb transcript was detected only at early developmental stages in the two brain areas. Correlating the temporal expression of the 9\u2010kb mRNA to that of high\u2010molecular\u2010weight MAP2 indicates that the accumulation of this protein is in part regulated at a cytoplasmic level.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1991.tb08163.x","type":"journal-article","created":{"date-parts":[[2006,10,6]],"date-time":"2006-10-06T01:18:32Z","timestamp":1160097512000},"page":"385-391","source":"Crossref","is-referenced-by-count":38,"title":["Expression of Various Microtubule\u2010Associated Protein 2 Forms in the Developing Mouse Brain and in Cultured Neurons and Astrocytes"],"prefix":"10.1111","volume":"56","author":[{"given":"C\u00e9cile","family":"Charri\u00e8re\u2010Bertrand","sequence":"first","affiliation":[]},{"given":"Craig","family":"Garner","sequence":"additional","affiliation":[]},{"given":"Marcienne","family":"Tardy","sequence":"additional","affiliation":[]},{"given":"Jacques","family":"Nunez","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450305"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450402"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901450403"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb06030.x"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1408"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902260205"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.17.5613"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(88)90013-9"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(84)90220-3"},{"key":"e_1_2_2_11_1","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1523\/JNEUROSCI.04-02-00394.1984","article-title":"Differential sublocalization of tubulin and the microtubule\u2010associated protein MAP2 in brain tissue as revealed by immunochemistry with monoclonal hybridoma antibodies","volume":"4","author":"Caceres A.","year":"1984","journal-title":"J. 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