{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:30:47Z","timestamp":1770431447957,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":4935,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1993,4]]},"abstract":"<jats:p><jats:bold>Abstract: <\/jats:bold> Using receptors expressed from mouse brain mRNA in <jats:italic>Xenopus<\/jats:italic> oocytes, we found that enhancement of type A \u03b3\u2010aminobutyric acid (GABA<jats:sub>A<\/jats:sub>) receptor\u2010gated Cl<jats:sup>\u2212<\/jats:sup> channel response is a common action of structurally diverse anesthetics, suggesting that the GABA<jats:sub>A<\/jats:sub> receptor plays an important role in anesthesia. To determine if GABA<jats:sub>A<\/jats:sub> receptor subunit composition influences actions of anesthetics, we expressed subunit cRNAs in <jats:italic>Xenopus<\/jats:italic> oocytes and measured effects of enflurane on GABA\u2010activated Cl<jats:sup>\u2212<\/jats:sup> currents. Potentiation of GABA\u2010activated currents by enflurane was dependent on the composition of GABA<jats:sub>A<\/jats:sub> receptor protein subunits; the order of sensitivity was \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> &gt; \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>\u03b3<jats:sub>2s<\/jats:sub>=\u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>\u03b3<jats:sub>2L<\/jats:sub> &gt; total mRNA. The results suggest that anesthetics with simple structures may act on the GABA<jats:sub>A<\/jats:sub> receptor protein complex to modulate the Cl<jats:sup>\u2212<\/jats:sup> channel activity and provide a molecular explanation for the synergistic clinical interactions between benzodiazepines and general anesthetics.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1993.tb03320.x","type":"journal-article","created":{"date-parts":[[2006,10,6]],"date-time":"2006-10-06T04:12:53Z","timestamp":1160107973000},"page":"1548-1553","source":"Crossref","is-referenced-by-count":41,"title":["Molecular Determinants of General Anesthetic Action: Role of GABA<sub>A<\/sub> Receptor Structure"],"prefix":"10.1111","volume":"60","author":[{"given":"Lie\u2010Huey","family":"Lin","sequence":"first","affiliation":[]},{"given":"Paul","family":"Whiting","sequence":"additional","affiliation":[]},{"given":"R. Adron","family":"Harris","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198508000-00005"},{"key":"e_1_2_2_3_1","first-page":"1343","article-title":"Propofol, a novel general anesthetic, has specific recognition sites in the rat brain","volume":"21","author":"Concas A.","year":"1991","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_2_4_1","first-page":"E97","article-title":"Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose\u2010response curves","volume":"235","author":"Lean A.","year":"1978","journal-title":"Am. J. 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