{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T11:06:11Z","timestamp":1749121571528},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T00:00:00Z","timestamp":1342656000000},"content-version":"vor","delay-in-days":11583,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1980,11]]},"abstract":"<jats:p><jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of the ionophore, A23187, on the intra\u2010axonal transport of dopamine \u03b2\u2010hydroxylase (DBH) were investigated in the cat hypogastric nerve\u2010inferior mesenteric ganglion preparation by monitoring, <jats:italic>in vitro<\/jats:italic>, the enzyme accumulation above a ligature, 2 to 2.5 cm distal to the ganglion.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>DBH accumulation in the proximal segment immediately above the ligature (P<jats:sub>1<\/jats:sub>) increased linearly up to 6 h, during incubation in normal Krebs solution at 37\u00b0C. The ionophore, A23187, interfered with the enzyme accumulation, but did not modify the previously accumulated DBH activity present in P<jats:sub>1<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The blocking effects of A23187 on DBH transport were greatly impaired in the absence of extracellular calcium ions; an excess of calcium in the bathing solution (7.5 mM) itself blocked the enzyme transport by 50%.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A23187 did not significantly modify the levels of adenosine triphosphate (ATP) in the segments P<jats:sub>1<\/jats:sub> and P<jats:sub>2<\/jats:sub> of the nerve proximal to the ligature.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Nerves incubated in an A23187\u2010containing medium showed many mitochondria of normal shape and fine structure; however, typical microtubules or filaments were not seen in these preparations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that the ionophore A23187, by considerably raising the axoplasmic ionized calcium levels, interferes with the assembling of microtubules. In this manner, the ionophore would inhibit the transport of adrenergic vesicles and therefore of DBH along the axon. The results also provide additional evidence in favour of the view that for the transport system to work adequately, it is necessary to maintain the intra\u2010axoplasmic ionized calcium concentration between certain critical levels.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1111\/j.1476-5381.1980.tb08713.x","type":"journal-article","created":{"date-parts":[[2012,7,20]],"date-time":"2012-07-20T10:20:07Z","timestamp":1342779607000},"page":"375-381","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["THE EFFECTS OF THE CALCIUM IONOPHORE, A23187, ON THE AXOPLASMIC TRANSPORT OF DOPAMINE \u03b2\u2010HYDROXYLASE"],"prefix":"10.1111","volume":"70","author":[{"given":"E.","family":"ESQUERRO","sequence":"first","affiliation":[]},{"given":"A.G.","family":"GARCIA","sequence":"additional","affiliation":[]},{"given":"P.","family":"SANCHEZ\u2010GARCIA","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2012,7,19]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"349","volume-title":"Concanavalin A as a Tool.","author":"Aunis D.","year":"1976"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-018298-8.50005-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1969.sp008720"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009544"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1973.sp010144"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.34.2.525"},{"key":"e_1_2_1_8_1","first-page":"209","volume-title":"Advances in Cytopharmacology","author":"Carafoli E.","year":"1971"},{"key":"e_1_2_1_9_1","first-page":"20","volume-title":"Bayer\u2010symposium II","author":"Dahlstr\u00f6m A.","year":"1970"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1971.0064"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.61.3.812"},{"key":"e_1_2_1_12_1","first-page":"213","volume-title":"Effects of calcium ionophores on the axonal transport of dopamine \u03b2\u2010hydroxylase (DBH)","author":"Esquerro E.","year":"1977"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(75)90164-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010686"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1152\/physrev.1971.51.1.98","article-title":"Synaptic vesicles in sympathetic neurones","volume":"51","author":"Geffen L.B.","year":"1971","journal-title":"Physiol. 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