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One minicircle from isolate 30T was cloned and sequenced, showing low levels of homology but the same general features and organization as described for minicircles of other trypanosomatids. Extensive regions of the minicircle are composed by G and T on the H strand. These regions are very repetitive and similar to regions in a minicircle of <jats:italic>Crithidia oncopelti<\/jats:italic> and to telomeric sequences of <jats:italic>Saccharomyces cerevisiae.<\/jats:italic> Conserved Sequence Block 3, present in all trypanosomatids, is one nucleotide different from the consensus in <jats:italic>P. serpens<\/jats:italic> and provides a basis to differentiate <jats:italic>P. serpens<\/jats:italic> from other trypanosomatids. Electron microscopy of kinetoplast DNA evidenced a network with organization similar to other trypanosomatids and the measurement of minicircles confirmed the size of about 1.45 kb of the sequenced minicircle.<\/jats:p>","DOI":"10.1111\/j.1550-7408.1993.tb04922.x","type":"journal-article","created":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T05:46:47Z","timestamp":1179121607000},"page":"317-322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characterization of Kinetoplast DNA from Phytomonas serpens"],"prefix":"10.1111","volume":"40","author":[{"given":"DANIEL","family":"S\u00c1\u2010CARVALHO","sequence":"first","affiliation":[]},{"given":"DAVID","family":"PEREZ\u2010MORGA","sequence":"additional","affiliation":[]},{"given":"YARA M.","family":"TRAUB\u2010CSEKO","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2007,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0248-4900(90)90374-C"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(81)90076-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(90)90388-K"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1987.tb03209.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1989.tb05537.x"},{"key":"e_1_2_1_7_2","first-page":"D413","volume-title":"Methods in Enzymology","author":"Davis R. 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