{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T10:14:39Z","timestamp":1695636879716},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,2,7]],"date-time":"2006-02-07T00:00:00Z","timestamp":1139270400000},"content-version":"vor","delay-in-days":2655,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:p><jats:bold>ABSTRACT:<\/jats:bold> It has been shown that quinine, a bitter tasting substance, induces a cationic membrane current in frog taste receptor cells. We analyzed the quinine\u2010induced current fluctuation to further characterize the cationic channel conducting the current. Taste cells were enzymatically isolated from fungiform papillae of the bullfrog tongue. Membrane currents were recorded under voltage\u2010clamp by using the whole\u2010cell patch clamp technique. A Cs<jats:sup>+<\/jats:sup>\u2010containing pipette solution was used to block K<jats:sup>+<\/jats:sup> currents. When quinine was bath applied, it induced a steady inward current in cells held at \u201060 mV. The current data were digitized at a sampling frequency of 2 kHz after having been low\u2010pass filtered (cut\u2010off frequency, 1 kHz). The spectral density distribution was calculated by 1024 points fast Fourier transform (FFT), and was fitted by a sum of two Lorentzian functions, with corner frequencies at 10\u201020 Hz and at 90\u2010120 Hz. The single\u2010channel conduc tance calculated from the variance versus mean current plot was 5 pS in a normal saline solution containing 1.8 mM Ca<jats:sup>2+<\/jats:sup>. In a nominally Ca<jats:sup>2+<\/jats:sup>\u2010free solution, the single\u2010channel current was doubled. Our pre vious study has shown that the quinine\u2010induced current is enhanced in low extracellular [Ca<jats:sup>2+<\/jats:sup>]. This enhancement could be partly attributable to the Ca2+ suppression of the single\u2010channel conductance.<\/jats:p>","DOI":"10.1111\/j.1749-6632.1998.tb10557.x","type":"journal-article","created":{"date-parts":[[2006,2,7]],"date-time":"2006-02-07T09:34:33Z","timestamp":1139304873000},"page":"148-149","source":"Crossref","is-referenced-by-count":2,"title":["Noise Analysis of the Quinine\u2010Induced Current in Frog Taste Receptor Cells<sup>a<\/sup>"],"prefix":"10.1111","volume":"855","author":[{"given":"TAKASHI","family":"TSUNENARI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AKIMICHI","family":"KANEKO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,7]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.108.6.515"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/331351a0"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374325"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199310000-00002"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/376085a0"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1749-6632.1998.tb10557.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1749-6632.1998.tb10557.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:33:59Z","timestamp":1695634439000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1749-6632.1998.tb10557.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["10.1111\/j.1749-6632.1998.tb10557.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1749-6632.1998.tb10557.x","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,11]]}}}