{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:33:17Z","timestamp":1758925997735},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,24]],"date-time":"2006-01-24T00:00:00Z","timestamp":1138060800000},"content-version":"vor","delay-in-days":999,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>:<\/jats:bold>A major problem hampering the development of effective stem cell\u2010based therapies is the absence of a clear understanding of the composition of the hematopoietic stem cell (HSC) pool in humans and how<jats:italic>ex vivo<\/jats:italic>manipulation can differentially affect the various HSC classes. This paper will review recent advances in the use of the NOD\/SCID xenotransplant assay to characterize the human stem cell compartment and to determine how<jats:italic>ex vivo<\/jats:italic>culture affects stem cells. Using lentivector\u2010mediated clonal tracking we found that only 4 days of culture can significantly reduce the number of SCID\u2010repopulating cells (SRCs) contributing to the human graft. Similar results were seen with a competitive assay strategy where non\u2010cultured cells marked with the RFP\u2010lentivector markedly outcompete cultured cells marked with a EGFP\u2010lentivector both transplanted into the same NOD\/SCID mouse. A novel intrafemoral (IF) assay was developed to permit the transplantation of human stem cells that might be difficult to detect using the traditional IV injection method. With the IF assay we identified a novel class of human stem cell with the ability to rapidly generate a large graft of human myeloid and erythroid cells within 2 weeks post transplant.<\/jats:p>","DOI":"10.1111\/j.1749-6632.2003.tb03234.x","type":"journal-article","created":{"date-parts":[[2009,3,6]],"date-time":"2009-03-06T14:38:02Z","timestamp":1236350282000},"page":"67-71","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of Cord Blood Hematopoietic Stem Cells"],"prefix":"10.1111","volume":"996","author":[{"given":"FR\u00c9D\u00c9RIC","family":"MAZURIER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MONICA","family":"DOEDENS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OLGA I.","family":"GAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOHN E.","family":"DICK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,24]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"crossref","unstructured":"Wang J.C. C. Dorrell C.Y. Ito et al.2001. Normal and leukemic human stem cells assayed in immune\u2010deficient mice.InHematopoiesis: A Developmental Approach. L.I. Zon Ed.: 99\u2013118. Oxford University Press. New York.","DOI":"10.1093\/oso\/9780195124507.003.0008"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/83199"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(02)00883-4"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5403.845"},{"key":"e_1_2_2_6_2","doi-asserted-by":"crossref","first-page":"3289","DOI":"10.1182\/blood.V95.11.3289","article-title":"The chemokine SDF\u20101 activates the integrins LFA\u20101, VLA\u20104, and VLA\u20105 on immature human CD34(+) cells: role in transendothelial\/stromal migration and engraftment of NOD\/SCID mice [In Process Citation]","volume":"95","author":"Peled A.","year":"2000","journal-title":"Blood"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11519"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5320"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9836"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/2023"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.7.3692"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012336799"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1749-6632.2003.tb03234.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1749-6632.2003.tb03234.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T04:27:34Z","timestamp":1710131254000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1749-6632.2003.tb03234.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1111\/j.1749-6632.2003.tb03234.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1749-6632.2003.tb03234.x","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5]]}}}