{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:35:52Z","timestamp":1770881752579,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T00:00:00Z","timestamp":1416787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB766"],"award-info":[{"award-number":["SFB766"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000614","name":"Federation of European Microbiological Societies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000614","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Max Plack Society","award":["institutional"],"award-info":[{"award-number":["institutional"]}]},{"name":"UKT Fortune Program","award":["F1433253"],"award-info":[{"award-number":["F1433253"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ntimin and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>nvasin are prototypical inverse (<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>ype <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>e) autotransporters and important virulence factors of enteropathogenic <jats:styled-content style=\"fixed-case\"><jats:italic>E<\/jats:italic><\/jats:styled-content><jats:italic>scherichia coli<\/jats:italic> and <jats:styled-content style=\"fixed-case\"><jats:italic>Y<\/jats:italic><\/jats:styled-content><jats:italic>ersinia spp<\/jats:italic>. respectively. In addition to a <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010terminal extracellular domain and a \u03b2\u2010barrel transmembrane domain, both proteins also contain a short <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal periplasmic domain that, in <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ntimin, includes a lysin motif (<jats:styled-content style=\"fixed-case\">LysM<\/jats:styled-content>), which is thought to mediate binding to peptidoglycan. We show that the periplasmic domain of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ntimin does bind to peptidoglycan both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, but only under acidic conditions. We were able to determine a dissociation constant of 0.8\u2009\u03bcM for this interaction, whereas the <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>nvasin periplasmic domain, which lacks a <jats:styled-content style=\"fixed-case\">LysM<\/jats:styled-content>, bound only weakly <jats:italic>in vitro<\/jats:italic> and failed to bind peptidoglycan <jats:italic>in vivo<\/jats:italic>. We present the solution structure of the <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ntimin <jats:styled-content style=\"fixed-case\">LysM<\/jats:styled-content>, which has an additional \u03b1\u2010helix conserved within inverse autotransporter <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>ys<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>s but lacking in others. In contrast to previous reports, we demonstrate that the periplasmic domain of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ntimin mediates dimerisation. We further show that dimerisation and peptidoglycan binding are general features of <jats:styled-content style=\"fixed-case\">LysM<\/jats:styled-content>\u2010containing inverse autotransporters. Peptidoglycan binding by the periplasmic domain in the infection process may aid in resisting mechanical and chemical stress during transit through the gastrointestinal tract.<\/jats:p>","DOI":"10.1111\/mmi.12840","type":"journal-article","created":{"date-parts":[[2014,10,29]],"date-time":"2014-10-29T09:28:45Z","timestamp":1414574925000},"page":"80-100","source":"Crossref","is-referenced-by-count":33,"title":["The <scp>I<\/scp>ntimin periplasmic domain mediates dimerisation and binding to peptidoglycan"],"prefix":"10.1111","volume":"95","author":[{"given":"Jack C.","family":"Leo","sequence":"first","affiliation":[{"name":"Department of Protein Evolution Max Planck Institute for Developmental Biology  72076 T\u00fcbingen Germany"}]},{"given":"Philipp","family":"Oberhettinger","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Microbiology and Infection Medicine University Clinics T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Manish","family":"Chaubey","sequence":"additional","affiliation":[{"name":"Department of Protein Evolution Max Planck Institute for Developmental Biology  72076 T\u00fcbingen Germany"}]},{"given":"Monika","family":"Sch\u00fctz","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Microbiology and Infection Medicine University Clinics T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Daniel","family":"K\u00fchner","sequence":"additional","affiliation":[{"name":"Department of Microbial Genetics University of T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Ute","family":"Bertsche","sequence":"additional","affiliation":[{"name":"Department of Microbial Genetics University of T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Heinz","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Protein Evolution Max Planck Institute for Developmental Biology  72076 T\u00fcbingen Germany"}]},{"given":"Friedrich","family":"G\u00f6tz","sequence":"additional","affiliation":[{"name":"Department of Microbial Genetics University of T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Ingo B.","family":"Autenrieth","sequence":"additional","affiliation":[{"name":"Interfaculty Institute for Microbiology and Infection Medicine University Clinics T\u00fcbingen  72076 T\u00fcbingen Germany"}]},{"given":"Murray","family":"Coles","sequence":"additional","affiliation":[{"name":"Department of Protein Evolution Max Planck Institute for Developmental Biology  72076 T\u00fcbingen Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-6752","authenticated-orcid":false,"given":"Dirk","family":"Linke","sequence":"additional","affiliation":[{"name":"Department of Protein Evolution Max Planck Institute for Developmental Biology  72076 T\u00fcbingen Germany"},{"name":"Department of Biosciences University of Oslo  0316 Oslo 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