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However, how these proteins without a typical signal peptide are excreted in bacteria is poorly understood. We studied the excretion pattern of cytoplasmic proteins using two glycolytic model enzymes, aldolase and enolase, and show that their excretion takes place mainly during the exponential growth phase in <jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>taphylococcus aureus<\/jats:italic> very similar to that of <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>bi, an <jats:styled-content style=\"fixed-case\">IgG<\/jats:styled-content>\u2010binding protein, which is secreted via the <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ec\u2010pathway. The amount of excreted enolase is substantial and is comparable with that of <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>bi. For localization of the exit site, we fused aldolase and enolase with the peptidoglycan\u2010binding motif, <jats:styled-content style=\"fixed-case\">LysM<\/jats:styled-content>, to trap the enzymes at the cell wall. With both immune fluorescence labeling and immunogold localization on electron microscopic thin sections aldolase and enolase were found apart from the cytoplasmic area particularly in the cross wall and at the septal cleft of dividing cells, whereas the non\u2010excreted <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>dh2, a soluble <jats:styled-content style=\"fixed-case\">NADH<\/jats:styled-content>:quinone oxidoreductase, is only seen attached to the inner side of the cytoplasmic membrane. The selectivity, the timing and the localization suggest that <jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content> is not a result of unspecific cell lysis but is mediated by an as yet unknown mechanism.<\/jats:p>","DOI":"10.1111\/mmi.13065","type":"journal-article","created":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T02:55:31Z","timestamp":1432608931000},"page":"775-789","source":"Crossref","is-referenced-by-count":58,"title":["Excretion of cytoplasmic proteins (<scp>ECP<\/scp>) in <scp><i>S<\/i><\/scp><i>taphylococcus aureus<\/i>"],"prefix":"10.1111","volume":"97","author":[{"given":"Patrick","family":"Ebner","sequence":"first","affiliation":[{"name":"Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine (IMIT) University of T\u00fcbingen  Auf der Morgenstelle 28 72076 T\u00fcbingen Germany"}]},{"given":"Marcel","family":"Prax","sequence":"additional","affiliation":[{"name":"Microbial Genetics, Interfaculty Institute of 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