{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:56:03Z","timestamp":1792202163647,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T00:00:00Z","timestamp":1393286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>An abnormal accumulation of cholesterol oxidation products in the brain of patients with Alzheimer's disease (<jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>) would further link an impaired cholesterol metabolism in the pathogenesis of the disease. The first evidence stemming from the content of oxysterols in autopsy samples from <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> and normal brains points to an increase in both 27\u2010hydroxycholesterol (27\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>) and 24\u2010hydroxycholesterol (24\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>) in the frontal cortex of <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> brains, with a trend that appears related to the disease severity. The challenge of differentiated <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>\u2010N\u2010<jats:styled-content style=\"fixed-case\">BE<\/jats:styled-content> human neuroblastoma cells with patho\u2010physiologically relevant amounts of 27\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> and 24\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content> showed that both oxysterols induce a net synthesis of A\u03b2<jats:sub>1\u201042<\/jats:sub> by up\u2010regulating expression levels of amyloid precursor protein and \u03b2\u2010secretase, as well as the \u03b2\u2010secretase activity. Interestingly, cell pretreatment with <jats:italic>N<\/jats:italic>\u2010acetyl\u2010cysteine (<jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content>) fully prevented the enhancement of \u03b2\u2010amyloidogenesis induced by the two oxysterols. The reported findings link an impaired cholesterol oxidative metabolism to an excessive \u03b2\u2010amyloidogenesis and point to <jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content> as an efficient inhibitor of oxysterols\u2010induced A\u03b2 toxic peptide accumulation in the brain.<\/jats:p>","DOI":"10.1111\/acel.12206","type":"journal-article","created":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T11:02:48Z","timestamp":1393326168000},"page":"561-572","source":"Crossref","is-referenced-by-count":59,"title":["Up\u2010regulation of \u03b2\u2010amyloidogenesis in neuron\u2010like human cells by both 24\u2010 and 27\u2010hydroxycholesterol: protective effect of <i>N<\/i>\u2010acetyl\u2010cysteine"],"prefix":"10.1111","volume":"13","author":[{"given":"Paola","family":"Gamba","sequence":"first","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Guglielmotto","sequence":"additional","affiliation":[{"name":"Department of Neuroscience \u201cRita Levi Montalcini\u201d University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Testa","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debora","family":"Monteleone","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"},{"name":"Department of Neuroscience \u201cRita Levi Montalcini\u201d University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Zerbinati","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnology Vascular Biology and Mass Spectrometry Laboratory Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Gargiulo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiorella","family":"Biasi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Iuliano","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnology Vascular Biology and Mass Spectrometry Laboratory Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Giaccone","sequence":"additional","affiliation":[{"name":"Foundation IRCCS Institute of Neurology Carlo Besta Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Mauro","sequence":"additional","affiliation":[{"name":"Division of Neurology and Neurorehabilitation IRCCS Italian Institute of Auxology Verbania Italy"},{"name":"Department of Neurosciences University of Turin Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Poli","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Tamagno","sequence":"additional","affiliation":[{"name":"Department of Neuroscience \u201cRita Levi Montalcini\u201d University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Leonarduzzi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences University of Turin Orbassano Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200506210-00007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2013.01.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.09.020"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.03.024"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000120374.59826.1b"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.02.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00308809"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.02.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402324200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1995.65020537.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1503\/jpn.100057"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2012.07.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000125323.15458.3F"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.05.033"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2011.00681.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06513.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2008-15412"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.03.019"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M300320-JLR200"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2012.12.023"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2011.06.006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00467-0"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3233\/JAD-2009-0944","article-title":"Impact of 27\u2010hydroxycholesterol on amyloid\u2010beta peptide production and ATP\u2010binding cassette transporter expression in primary human neurons","volume":"16","author":"Kim WS","year":"2009","journal-title":"J. Alzheimers Dis."},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(02)00222-X"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3656"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.24.10703-10717.2004"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.02.003"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2012.12.001"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-4-1"},{"key":"e_1_2_7_33_1","first-page":"271","article-title":"Influence of LOX\/COX inhibitors on cell differentiation induced by all\u2010trans retinoic acid in neuroblastoma cell lines","volume":"25","author":"Redova M","year":"2010","journal-title":"Int. J. Mol. Med."},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2011-110599"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1091"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.2217\/fnl.12.27"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867013372922"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)00164-7"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.3233\/JAD-2011-110854","article-title":"Processing of endogenous A\u03b2PP in blood\u2010brain barrier endothelial cells is modulated by liver\u2010X receptor agonists and altered cellular cholesterol homeostasis","volume":"27","author":"Schweinzer C","year":"2011","journal-title":"J. Alzheimers Dis."},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e32830b8ae1"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03667-0"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986709787458353"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-9961(03)00131-1"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.08.581"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3569"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.58.12.1791"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-231456"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.02.006"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.09.010"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facel.12206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.12206","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.12206","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.12206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T02:01:56Z","timestamp":1696471316000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.12206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,25]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/acel.12206"],"URL":"https:\/\/doi.org\/10.1111\/acel.12206","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,25]]}}}