{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T21:56:53Z","timestamp":1789595813047,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T00:00:00Z","timestamp":1504742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Female middle age is characterized by a decline in skeletal muscle mass and performance, predisposing women to sarcopenia, functional limitations, and metabolic dysfunction as they age. Menopausal loss of ovarian function leading to low circulating level of 17\u03b2\u2010estradiol has been suggested as a contributing factor to aging\u2010related muscle deterioration. However, the underlying molecular mechanisms remain largely unknown and thus far androgens have been considered as a major anabolic hormone for skeletal muscle. We utilized muscle samples from 24 pre\u2010 and postmenopausal women to establish proteome\u2010wide profiles, associated with the difference in age (30\u201334\u00a0years old vs. 54\u201362\u00a0years old), menopausal status (premenopausal vs. postmenopausal), and use of hormone replacement therapy (<jats:styled-content style=\"fixed-case\">HRT<\/jats:styled-content>; user vs. nonuser). None of the premenopausal women used hormonal medication while the postmenopausal women were monozygotic (<jats:styled-content style=\"fixed-case\">MZ<\/jats:styled-content>) cotwin pairs of whom the other sister was current <jats:styled-content style=\"fixed-case\">HRT<\/jats:styled-content> user or the other had never used <jats:styled-content style=\"fixed-case\">HRT<\/jats:styled-content>. Label\u2010free proteomic analyses resulted in the quantification of 797 muscle proteins of which 145 proteins were for the first time associated with female aging using proteomics. Furthermore, we identified 17\u03b2\u2010estradiol as a potential upstream regulator of the observed differences in muscle energy pathways. These findings pinpoint the underlying molecular mechanisms of the metabolic dysfunction accruing upon menopause, thus having implications for understanding the complex functional interactions between female reproductive hormones and health.<\/jats:p>","DOI":"10.1111\/acel.12661","type":"journal-article","created":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T02:02:53Z","timestamp":1504836173000},"page":"1276-1287","source":"Crossref","is-referenced-by-count":60,"title":["Estrogenic regulation of skeletal muscle proteome: a study of premenopausal women and postmenopausal <scp>MZ<\/scp> cotwins discordant for hormonal therapy"],"prefix":"10.1111","volume":"16","author":[{"given":"Eija K.","family":"Laakkonen","sequence":"first","affiliation":[{"name":"Faculty of Sport and Health Sciences Gerontology Research Center University of Jyv\u00e4skyl\u00e4 Jyv\u00e4skyl\u00e4 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabah","family":"Soliymani","sequence":"additional","affiliation":[{"name":"Medicum, Biochemistry\/Developmental Biology Meilahti Clinical Proteomics Core Facility University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sira","family":"Karvinen","sequence":"additional","affiliation":[{"name":"Faculty of Sport and Health Sciences Gerontology Research Center University of Jyv\u00e4skyl\u00e4 Jyv\u00e4skyl\u00e4 Finland"},{"name":"Divisions of Rehabilitation Science and Physical Therapy Department of Rehabilitation Medicine Medical School University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaakko","family":"Kaprio","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine FIMM University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urho M.","family":"Kujala","sequence":"additional","affiliation":[{"name":"Faculty of Sport and Health Sciences University of Jyv\u00e4skyl\u00e4 Jyv\u00e4skyl\u00e4 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Baumann","sequence":"additional","affiliation":[{"name":"Medicum, Biochemistry\/Developmental Biology Meilahti Clinical Proteomics Core Facility University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarianna","family":"Sipil\u00e4","sequence":"additional","affiliation":[{"name":"Faculty of Sport and Health Sciences Gerontology Research Center University of Jyv\u00e4skyl\u00e4 Jyv\u00e4skyl\u00e4 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vuokko","family":"Kovanen","sequence":"additional","affiliation":[{"name":"Faculty of Sport and Health Sciences University of Jyv\u00e4skyl\u00e4 Jyv\u00e4skyl\u00e4 Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Lalowski","sequence":"additional","affiliation":[{"name":"Medicum, Biochemistry\/Developmental Biology Meilahti Clinical Proteomics Core Facility University of Helsinki Helsinki 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