{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T04:01:13Z","timestamp":1792468873656,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR065530"],"award-info":[{"award-number":["R01AR065530"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aging is a major risk factor of intervertebral disc degeneration and a leading cause of back pain. Pathological changes associated with disc degeneration include the absence of large, vacuolated and reticular\u2010shaped nucleus pulposus cells, and appearance of smaller cells nested in lacunae. These small nested cells are conventionally described as chondrocyte\u2010like cells; however, their origin in the intervertebral disc is unknown. Here, using a genetic mouse model and a fate mapping strategy, we have found that the chondrocyte\u2010like cells in degenerating intervertebral discs are, in fact, nucleus pulposus cells. With aging, the nucleus pulposus cells fuse their cell membranes to form the nested lacunae. Next, we characterized the expression of sonic hedgehog (SHH), crucial for the maintenance of nucleus pulposus cells, and found that as intervertebral discs age and degenerate, expression of SHH and its target Brachyury is gradually lost. The results indicate that the chondrocyte\u2010like phenotype represents a terminal stage of differentiation preceding loss of nucleus pulposus cells and disc collapse.<\/jats:p>","DOI":"10.1111\/acel.13006","type":"journal-article","created":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T10:05:47Z","timestamp":1562753147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Chondrocyte\u2010like nested cells in the aged intervertebral disc are late\u2010stage nucleus pulposus cells"],"prefix":"10.1111","volume":"18","author":[{"given":"Sarthak","family":"Mohanty","sequence":"first","affiliation":[{"name":"Hospital for Special Surgery New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Pinelli","sequence":"additional","affiliation":[{"name":"Hospital for Special Surgery New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Pricop","sequence":"additional","affiliation":[{"name":"Hospital for Special Surgery New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd J.","family":"Albert","sequence":"additional","affiliation":[{"name":"Hospital for Special Surgery New York NY USA"},{"name":"Weill Cornell Medical College New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3683-9791","authenticated-orcid":false,"given":"Chitra Lekha","family":"Dahia","sequence":"additional","affiliation":[{"name":"Hospital for Special Surgery New York NY USA"},{"name":"Weill Cornell Medical College New York NY USA"},{"name":"Department of Cell and Developmental Biology, Weill Cornell Medicine Graduate School of Medical Sciences New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.035592"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574888X10666150113112149"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21805"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0b013e3181913e98"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035944"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.05.018"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0021-8782.2004.00352.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22376"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.brs.0000162624.95262.73"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199501000-00004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2270545"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.BRS.0000061986.03886.4F"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20397"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1349400"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.160"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092040403"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(82)90033-7"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-010-1392-z"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098444"}],"container-title":["Aging 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