{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:23:29Z","timestamp":1790832209480,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&D Program of China","award":["2018YFC2000100"],"award-info":[{"award-number":["2018YFC2000100"]}]},{"name":"National Key R&D Program of China","award":["2017YFA0103300"],"award-info":[{"award-number":["2017YFA0103300"]}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["81622019"],"award-info":[{"award-number":["81622019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["31871438"],"award-info":[{"award-number":["31871438"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["31570813"],"award-info":[{"award-number":["31570813"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["81702770"],"award-info":[{"award-number":["81702770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["81702638"],"award-info":[{"award-number":["81702638"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["81601212"],"award-info":[{"award-number":["81601212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012247","name":"Program of Shanghai Academic Research Leader","doi-asserted-by":"crossref","award":["19XD1403000"],"award-info":[{"award-number":["19XD1403000"]}],"id":[{"id":"10.13039\/501100012247","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds for the Central Universities, the Open Project Program of State Key Laboratory of Natural Medicines","award":["SKLNMKF201905"],"award-info":[{"award-number":["SKLNMKF201905"]}]},{"name":"Shanghai municipal medical and health discipline construction projects","award":["2017ZZ02015"],"award-info":[{"award-number":["2017ZZ02015"]}]},{"name":"\"Shu Guang\" project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation","award":["19SG18"],"award-info":[{"award-number":["19SG18"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The decline in DNA repair capacity contributes to the age\u2010associated\u00a0decrease in genome\u00a0integrity in somatic cells of different species. However, due to the lack of clinical samples and appropriate tools for studying DNA repair, whether and how age\u2010associated changes in DNA repair result in a loss of genome integrity of human adult stem cells remains incompletely characterized. Here, we isolated 20 eyelid adipose\u2010derived stem cell (ADSC) lines from healthy individuals (young: 10 donors with ages ranging 17\u201325\u00a0years; old: 10 donors with ages ranging 50\u201359\u00a0years). Using these cell lines, we systematically compared the efficiency of base excision repair (BER) and two DNA double\u2010strand break (DSB) repair pathways\u2014nonhomologous end joining (NHEJ) and homologous recombination (HR)\u2014between the young and old groups. Surprisingly, we found that the efficiency of BER but not NHEJ or HR is impaired in aged human ADSCs, which is in contrast to previous findings that DSB repair declines with age in human fibroblasts. We also demonstrated that BER efficiency is negatively associated with tail moment, which reflects a loss of genome integrity in human ADSCs. Mechanistic studies indicated that at the protein level XRCC1, but not other BER factors, exhibited age\u2010associated decline. Overexpression of XRCC1 reversed the decline of BER efficiency and genome integrity, indicating that XRCC1 is a potential therapeutic target for stabilizing genomes in aged ADSCs.<\/jats:p>","DOI":"10.1111\/acel.13062","type":"journal-article","created":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T12:29:03Z","timestamp":1575030543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Base excision repair but not DNA double\u2010strand break repair is impaired in aged human adipose\u2010derived stem cells"],"prefix":"10.1111","volume":"19","author":[{"given":"Haiping","family":"Zhang","sequence":"first","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bailian","family":"Cai","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"},{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"Geng","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanyin","family":"Tang","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery Changzheng Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Jiang","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Tan","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine Xiangya Hospital Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Wan","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"},{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5298-1918","authenticated-orcid":false,"given":"Zhiyong","family":"Mao","sequence":"additional","affiliation":[{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and Technology Tongji University Shanghai China"},{"name":"Clinical and Translational Research Center of Shanghai First Maternity &amp; Infant Hospital School of Medicine Tongji University Shanghai China"},{"name":"State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111919109"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2015.01.004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.1.68"},{"key":"e_1_2_5_5_1","first-page":"e53886","article-title":"Isolation and Differentiation of Adipose\u2010Derived Stem Cells from Porcine Subcutaneous Adipose Tissues","volume":"109","author":"Chen Y. J.","year":"2016","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0344-z"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400127"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6513"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab3388"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1634"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108799108"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.4061\/2010\/543531"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.277"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2016.65"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689717721221"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-2197.2009.04852.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.09312"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200583109"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101390"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.12.027"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.243"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30137-4"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05862"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400726101"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004511"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1615497"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.08.004"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.4161\/15384101.2014.980641"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.12.002"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Facel.13062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.13062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.13062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.13062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T21:39:40Z","timestamp":1693863580000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.13062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,29]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/acel.13062"],"URL":"https:\/\/doi.org\/10.1111\/acel.13062","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,29]]},"assertion":[{"value":"2019-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13062"}}