{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T03:34:48Z","timestamp":1775187288234,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T00:00:00Z","timestamp":1617062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006578","name":"Chi Mei Medical Center","doi-asserted-by":"publisher","award":["CMFHT10802"],"award-info":[{"award-number":["CMFHT10802"]}],"id":[{"id":"10.13039\/501100006578","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Amyloid\u2010beta (A\u03b2) oligomer is known to contribute to the pathophysiology of age\u2010related macular degeneration. Herein, we aimed to elucidate the\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    effects of A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    application on retinal morphology in rats. Our\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies revealed that intracerebroventricular administration of A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    oligomer caused dysmorphological changes in both retinal ganglion cells and retinal pigment epithelium. In addition,\n                    <jats:italic>in vitro<\/jats:italic>\n                    studies revealed that ARPE\u201019 cells following A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    oligomer application had decreased viability along with apoptosis and decreased expression of the tight junction proteins, increased expression of both phosphor\u2010AKT and phosphor\u2010GSK3\u03b2 and decreased expression of both SIRT1 and \u03b2\u2010catenin. Application of conditioned medium (CM) obtained from mesenchymal stem cells (MSC) protected against A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    oligomer\u2010induced retinal pathology in both rats and ARPE\u201019 cells. In order to explore the potential role of peptides secreted from the MSCs, we applied mass spectrometry to compare the peptidomics profiles of the MSC\u2010CM. Gene ontology enrichment analysis and String analysis were performed to explore the differentially expressed peptides by predicting the functions of their precursor proteins. Bioinformatics analysis showed that 3\u20108 out of 155\u2013163 proteins in the MSC\u2010CM maybe associated with SIRT1\/pAKT\/pGSK3\u03b2\/\u03b2\u2010catenin, tight junction proteins, and apoptosis pathway. In particular, the secretomes information on the MSC\u2010CM may be helpful for the prevention and treatment of retinal pathology in age\u2010related macular degeneration.\n                  <\/jats:p>","DOI":"10.1111\/acel.13340","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T10:48:37Z","timestamp":1617101317000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mesenchymal stem cell\u2010conditioned medium attenuates the retinal pathology in amyloid\u2010\u03b2\u2010induced rat model of Alzheimer's disease: Underlying mechanisms"],"prefix":"10.1111","volume":"20","author":[{"given":"Shu\u2010Chun","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Chi Mei Medical Center  Tainan Taiwan"},{"name":"Department of Optometry Chung Hwa University of Medical Technology  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung\u2010Ching","family":"Chio","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery Department of Surgery Chi Mei Medical Center  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao\u2010Hung","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Optometry Chung Hwa University of Medical Technology  Tainan Taiwan"},{"name":"Division of Neurosurgery Department of Surgery Chi Mei Medical Center  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui\u2010Ti","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Medical Research Chi Mei Medical Center  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Pin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Research Chi Mei Medical Center  Tainan 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