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The frequency of chromosome segregation errors in oocytes increases with maternal age, especially at a late stage of reproductive life. How aging at various life stages affects oocytes differently remains poorly understood. In this study, we describe aging\u2010associated changes in the transcriptome profile of mouse oocytes throughout reproductive life. Our single\u2010oocyte comprehensive RNA sequencing using RamDA\u2010seq revealed that oocytes undergo transcriptome changes at a late reproductive stage, whereas their surrounding cumulus cells exhibit transcriptome changes at an earlier stage. Calorie restriction, a paradigm that reportedly prevents aging\u2010associated egg aneuploidy, promotes a transcriptome shift in oocytes with the up\u2010regulation of genes involved in chromosome segregation. This shift is accompanied by the improved maintenance of chromosomal cohesin, the loss of which is a hallmark of oocyte aging and causes chromosome segregation errors. These findings have implications for understanding how oocytes undergo aging\u2010associated functional decline throughout their reproductive life in a context\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/acel.13428","type":"journal-article","created":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T08:46:26Z","timestamp":1625906786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Single\u2010oocyte transcriptome analysis reveals aging\u2010associated effects influenced by life stage and calorie restriction"],"prefix":"10.1111","volume":"20","author":[{"given":"Tappei","family":"Mishina","sequence":"first","affiliation":[{"name":"Laboratory for Chromosome Segregation RIKEN Center for Biosystems Dynamics Research (BDR) Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namine","family":"Tabata","sequence":"additional","affiliation":[{"name":"Laboratory for Chromosome Segregation RIKEN Center for Biosystems Dynamics Research (BDR) Kobe Japan"},{"name":"Graduate School of Biostudies Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsutaro","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Laboratory for Bioinformatics Research RIKEN Center for Biosystems Dynamics Research (BDR) Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Laboratory for Bioinformatics Research RIKEN Center for Biosystems Dynamics Research (BDR) Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mana","family":"Umeda","sequence":"additional","affiliation":[{"name":"Laboratory for Bioinformatics Research RIKEN Center for Biosystems Dynamics Research (BDR) Kobe 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Biosystems Dynamics Research (BDR) Kobe Japan"},{"name":"Department of Functional Genome Informatics, Division of Medical Genomics, Medical Research Institute Tokyo Medical and Dental University (TMDU) Bunkyo Japan"},{"name":"Master's\/Doctoral Program in Life Science Innovation (Bioinformatics), Degree Programs in Systems and Information Engineering, Graduate School of Science and Technology University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6486-7143","authenticated-orcid":false,"given":"Tomoya S.","family":"Kitajima","sequence":"additional","affiliation":[{"name":"Laboratory for Chromosome Segregation RIKEN Center for Biosystems Dynamics Research (BDR) Kobe Japan"},{"name":"Graduate School of Biostudies Kyoto University Kyoto 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