{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:07:31Z","timestamp":1790024851703,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["F31 HD089597"],"award-info":[{"award-number":["F31 HD089597"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01 GM112081"],"award-info":[{"award-number":["R01 GM112081"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35 GM136340"],"award-info":[{"award-number":["R35 GM136340"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A hallmark of advanced maternal age is a significant increase in meiotic chromosome segregation errors, resulting in early miscarriages and congenital disorders. These errors most frequently occur during meiosis I (MI). The spindle assembly checkpoint (SAC) prevents chromosome segregation errors by arresting the cell cycle until proper chromosome alignment is achieved. Unlike in mitosis, the SAC in oocytes is desensitized, allowing chromosome segregation in the presence of improperly aligned chromosomes. Whether SAC integrity further deteriorates with advancing maternal age, and if this decline contributes to increased segregation errors remains a fundamental question. In somatic cells, activation of the SAC depends upon Aurora kinase B (AURKB), which functions to monitor kinetochore\u2013microtubule attachments and recruit SAC regulator proteins. In mice, oocyte\u2010specific deletion of AURKB (\n                    <jats:italic>Aurkb<\/jats:italic>\n                    cKO) results in an increased production of aneuploid metaphase II\u2010arrested eggs and premature age\u2010related infertility. Here, we aimed to understand the cause of the short reproductive lifespan and hypothesized that SAC integrity was compromised. In comparing oocytes from young and sexually mature\n                    <jats:italic>Aurkb<\/jats:italic>\n                    cKO females, we found that SAC integrity becomes compromised rapidly with maternal age. We show that the increased desensitization of the SAC is driven by reduced expression of MAD2, ZW10 and Securin proteins, key contributors to the SAC response pathway. The reduced expression of these proteins is the result of altered protein homeostasis, likely caused by the accumulation of reactive oxygen species. Taken together, our results demonstrate a novel function for AURKB in preserving the female reproductive lifespan possibly by protecting oocytes from oxidative stress.\n                  <\/jats:p>","DOI":"10.1111\/acel.13489","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T02:47:12Z","timestamp":1635302832000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Age\u2010dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B"],"prefix":"10.1111","volume":"20","author":[{"given":"Cecilia S.","family":"Blengini","sequence":"first","affiliation":[{"name":"Department of Genetics; Rutgers The State University of New Jersey Piscataway NJ USA"},{"name":"Human Genetics Institute of New Jersey Piscataway NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra L.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Genetics; Rutgers The State University of New Jersey Piscataway NJ USA"},{"name":"Human Genetics Institute of New Jersey Piscataway NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour","family":"Aboelenain","sequence":"additional","affiliation":[{"name":"Department of Genetics; Rutgers The State University of New Jersey Piscataway NJ USA"},{"name":"Human Genetics Institute of New Jersey Piscataway NJ USA"},{"name":"Department of Theriogenology Faculty of Veterinary Medicine Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6113-1184","authenticated-orcid":false,"given":"Karen","family":"Schindler","sequence":"additional","affiliation":[{"name":"Department of Genetics; Rutgers The State University of New Jersey Piscataway NJ USA"},{"name":"Human Genetics Institute of New Jersey Piscataway NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202000806"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38111"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03310-2"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8603-3_8"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9553"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.047"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.077909"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.066381"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082661"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.170272"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq014"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav7321"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.078352"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.328105"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204686109"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201902105"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.031757"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.077040"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12657"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.088583"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmw028"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06372-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.08.051"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15346"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3245"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.03.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.08.052"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gax018"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.01.048"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-019-01800-9"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.5.1301"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.11.125"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.10.006"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.70"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120517109"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.21398"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116783"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb06187.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.1.49"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/HUMREP\/10.6.1563"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02694-8"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.11.049"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.009"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.08.052"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201506020"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-019-0529-x"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-016-0693-5"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.09.006"}],"container-title":["Aging 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