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In particular, terminally differentiated CD8<jats:sup>+<\/jats:sup> effector memory CD45RA<jats:sup>+<\/jats:sup> TEMRA cells and their subsets have characteristics of cellular senescence, accumulate in older individuals, and are increased in age\u2010related chronic inflammatory diseases. In a detailed T\u2010cell profiling among individuals over 65\u00a0years of age, we found a high interindividual variation among CD8<jats:sup>+<\/jats:sup> TEMRA populations. CD8<jats:sup>+<\/jats:sup> TEMRA proportions correlated positively with cytomegalovirus (CMV) antibody levels, however, not with the chronological age. In the analysis of over 90 inflammation proteins, we identified plasma TRANCE\/RANKL levels to associate with several differentiated T\u2010cell populations, including CD8<jats:sup>+<\/jats:sup> TEMRA and its CD28<jats:sup>\u2212<\/jats:sup> subsets. Given the strong potential of CD8<jats:sup>+<\/jats:sup> TEMRA cells as a biomarker for immunosenescence, we used deep\u2010amplicon bisulfite sequencing to match their frequencies in flow cytometry with CpG site methylation levels and developed a computational model to predict CD8<jats:sup>+<\/jats:sup> TEMRA cell proportions from whole blood genomic DNA. Our findings confirm the association of CD8<jats:sup>+<\/jats:sup> TEMRA and its subsets with CMV infection and provide a novel tool for their high throughput epigenetic quantification as a biomarker of immunosenescence.<\/jats:p>","DOI":"10.1111\/acel.13607","type":"journal-article","created":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T17:45:31Z","timestamp":1649526331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Epigenetic quantification of immunosenescent CD8<sup>+<\/sup> TEMRA cells in human blood"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1528-3715","authenticated-orcid":false,"given":"Ahto","family":"Salumets","sequence":"first","affiliation":[{"name":"Molecular Pathology Institute of Biomedicine and Translational Medicine University of Tartu  Tartu Estonia"},{"name":"Institute of Computer Science University of Tartu  Tartu 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