{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:01:57Z","timestamp":1792612917413,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["5UG3TR002598"],"award-info":[{"award-number":["5UG3TR002598"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5UG3TR002598, Paul M. Coen: NIH\/NIA K01 AG044437"],"award-info":[{"award-number":["5UG3TR002598, Paul M. 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Following 17\u2009days differentiation including 5\u2009days of a 3\u00a0V, 2\u00a0Hz electrical stimulation regime, the myobundles exhibited fused myotube alignment and upregulation of myogenic, myofiber assembly, signaling and contractile genes as demonstrated by gene array profiling and localization of key components of the sarcomere. Our results demonstrate that myobundles derived from the young, active (YA) group showed high intensity immunofluorescent staining of \u03b1\u2010actinin proteins and responded to electrical stimuli with a ~1\u2009\u03bcm displacement magnitude compared with non\u2010stimulated myobundles. Myobundles derived from older sedentary group (OS) did not display a synchronous contraction response. Hypertrophic potential is increased in YA\u2010derived myobundles in response to stimulation as shown by upregulation of insulin growth factor (IGF\u20101), \u03b1\u2010actinin (ACTN3, ACTA1) and fast twitch troponin protein (TNNI2) compared with OS\u2010derived myobundles. Our MPS mimics disease states of muscle decline and thus provides an aged system and experimental platform to investigate electrical stimulation mimicking exercise regimes and may be adapted to long duration studies of compound efficacy and toxicity for therapeutic evaluation against sarcopenia.<\/jats:p>","DOI":"10.1111\/acel.13650","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T20:45:36Z","timestamp":1654202736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Microphysiological system for studying contractile differences in young, active, and old, sedentary adult derived skeletal muscle cells"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0772-8200","authenticated-orcid":false,"given":"Shelby","family":"Giza","sequence":"first","affiliation":[{"name":"Department of Pharmacodynamics, College of Pharmacy University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6350-1759","authenticated-orcid":false,"given":"Jorge A.","family":"Mojica\u2010Santiago","sequence":"additional","affiliation":[{"name":"J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7627-2729","authenticated-orcid":false,"given":"Maddalena","family":"Parafati","sequence":"additional","affiliation":[{"name":"Department of Pharmacodynamics, College of Pharmacy University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Legrand K.","family":"Malany","sequence":"additional","affiliation":[{"name":"Micro\u2010gRx, INC  Orlando Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don","family":"Platt","sequence":"additional","affiliation":[{"name":"Micro Aerospace Solutions  Melbourne Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5865-6016","authenticated-orcid":false,"given":"Christine E.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"J. 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