{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:20:35Z","timestamp":1790824835758,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007368","name":"Fondazione Pisa","doi-asserted-by":"publisher","award":["ETHERNA"],"award-info":[{"award-number":["ETHERNA"]}],"id":[{"id":"10.13039\/100007368","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parkinson's disease (PD) is characterized by phosphorylation and aggregation of the protein \u03b1\u2010Synuclein and ensuing neuronal death progressing from the noradrenergic <jats:italic>locus coeruleus<\/jats:italic> to midbrain dopaminergic neurons. In 2019, Matsui and colleagues reported a spontaneous age\u2010dependent degeneration of dopaminergic neurons and an even greater neurodegeneration of the noradrenergic neurons in the short\u2010lived killifish <jats:italic>Nothobranchius furzeri<\/jats:italic>. Given the great possible relevance of a spontaneous model for PD, we assessed neurodegeneration of noradrenergic and dopaminergic neurons in two further laboratory strains of <jats:italic>N. furzeri<\/jats:italic>. We implemented, for the first time in <jats:italic>N. furzeri,<\/jats:italic> a whole\u2010brain clarification technique and proceeded to entire 3D nuclei reconstruction to quantify total cell numbers in two different stains of <jats:italic>N. furzeri<\/jats:italic>. In both strains, we observed that age\u2010dependent neurodegeneration is limited to the <jats:italic>locus coeruleus<\/jats:italic> and does not involve the <jats:italic>posterior tuberculum<\/jats:italic>. We also applied 3D counting to the optic tectum, an area of active adult neurogenesis, and detected an increase of neurons with age. Our results confirm age\u2010dependent neurodegeneration of noradrenergic neurons, a condition reminiscent of the presymptomatic stage of PD indicating that <jats:italic>N. furzeri<\/jats:italic> could be used in the future to identify modifying factors for age\u2010dependent neurodegeneration and open the intriguing possibility that natural genetic variation may influence the susceptibility of dopaminergic neurons.<\/jats:p>","DOI":"10.1111\/acel.13689","type":"journal-article","created":{"date-parts":[[2022,8,20]],"date-time":"2022-08-20T06:41:36Z","timestamp":1660977696000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Quantification of noradrenergic\u2010, dopaminergic\u2010, and tectal\u2010neurons during aging in the short\u2010lived killifish <i>Nothobranchius furzeri<\/i>"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9491-5804","authenticated-orcid":false,"given":"Sara","family":"Bagnoli","sequence":"first","affiliation":[{"name":"Laboratory of Biology (BIO@SNS) Scuola Normale Superiore  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baldassare","family":"Fronte","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Bibbiani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Terzibasi Tozzini","sequence":"additional","affiliation":[{"name":"Biology and Evolution of Marine Organisms Dep. 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