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In the current study, we aimed to investigate sex and age differences in mitochondrial homeostasis, inflammation, and cellular senescence. Cardiac tissue samples from younger and older patients with inflammatory dilated cardiomyopathy (DCMI) were used. The expression of Sirt1, phosphorylated AMPK, PGC\u20101\u03b1, Sirt3, acetylated SOD2, catalase, and several mitochondrial genes was analyzed to assess mitochondrial homeostasis. The expression of NF\u2010\u03baB, TLR4, and interleukins was used to examine the inflammatory state in the heart. Finally, several senescence markers and telomere length were investigated. Cardiac AMPK expression and phosphorylation were significantly elevated in male DCMI patients, whereas Sirt1 expression remained unchanged in all groups investigated. AMPK upregulation was accompanied by a preserved expression of all mitochondrial proteins\/genes investigated in older male DCMI patients, whereas the expression of TOM40, TIM23, and the mitochondrial oxidative phosphorylation genes was significantly reduced in older female patients. Mitochondrial homeostasis in older male patients was further supported by the reduced acetylation of mitochondrial proteins as indicated by acetylated SOD2. The inflammatory markers NF\u2010\u03baB and TLR4 were downregulated in older male DCMI patients, whereas the expression of IL\u201018 was increased in older female patients. This was accompanied by progressed senescence in older DCMI hearts. In conclusion, older women experience more dramatic immunometabolic disorders on the cellular level than older men.<\/jats:p>","DOI":"10.1111\/acel.13894","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T02:43:55Z","timestamp":1687833835000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Sex and age differences in <scp>AMPK<\/scp> phosphorylation, mitochondrial homeostasis, and inflammation in hearts from inflammatory cardiomyopathy patients"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1391-9262","authenticated-orcid":false,"given":"Maria Luisa","family":"Barcena","sequence":"first","affiliation":[{"name":"Department of Geriatrics and Medical Gerontology Charit\u00e9 \u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin and Berlin Institute of Health  Berlin 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