{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:09:41Z","timestamp":1791083381458,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The age\u2010related decline in immunity reduces the effectiveness of vaccines in older adults. Immunosenescence is associated with chronic, low\u2010grade inflammation, and the accumulation of senescent cells. The latter express Bcl\u20102 family members (providing resistance to cell death) and exhibit a pro\u2010inflammatory, senescence\u2010associated secretory phenotype (SASP). Preexisting senescent cells cause many aging\u2010related disorders and therapeutic means of eliminating these cells have recently gained attention. The potential consequences of senescent cell removal on vaccine efficacy in older individuals are still ignored. We used the Bcl\u20102 family inhibitor ABT\u2010263 to investigate the effects of pre\u2010vaccination senolysis on immune responses in old mice. Two different ovalbumin (OVA)\u2010containing vaccines (containing a saponin\u2010based or a CpG oligodeoxynucleotide adjuvant) were tested. ABT\u2010263 depleted senescent cells (apoptosis) and ablated the basal and lipopolysaccharide\u2010induced production of SASP\u2010related factors in old mice. Depletion of senescent cells prior to vaccination (prime\/boost) had little effect on OVA\u2010specific antibody and T\u2010cell responses (slightly reduced and augmented, respectively). We then used a preclinical melanoma model to test the antitumor potential of senolysis before vaccination (prime with the vaccine and OVA boost by tumor cells). Surprisingly, ABT\u2010263 treatment abrogated the vaccine's ability to protect against B16 melanoma growth in old animals, an effect associated with reduced antigen\u2010specific T\u2010cell responses. Some, but not all, of the effects were age\u2010specific, which suggests that preexisting senescent cells were partly involved. Hence, depletion of senescent cells modifies immune responses to vaccines in some settings and caution should be taken when incorporating senolytics into vaccine\u2010based cancer therapies.<\/jats:p>","DOI":"10.1111\/acel.14007","type":"journal-article","created":{"date-parts":[[2023,11,25]],"date-time":"2023-11-25T01:34:53Z","timestamp":1700876093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Depletion of preexisting B\u2010cell lymphoma 2\u2010expressing senescent cells before vaccination impacts antigen\u2010specific antitumor immune responses in old mice"],"prefix":"10.1111","volume":"22","author":[{"given":"Ozmen","family":"Cobanoglu","sequence":"first","affiliation":[{"name":"CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 9017 \u2010 CIIL \u2010 Center for Infection and Immunity of Lille University of Lille  Lille 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