{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:39:10Z","timestamp":1790030350226,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["3 UH3 AR077748\u201002S1"],"award-info":[{"award-number":["3 UH3 AR077748\u201002S1"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["UH3AR077748"],"award-info":[{"award-number":["UH3AR077748"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001279","name":"Orthopaedic Research and Education Foundation","doi-asserted-by":"publisher","award":["N00014\u201019\u2010C\u20102052"],"award-info":[{"award-number":["N00014\u201019\u2010C\u20102052"]}],"id":[{"id":"10.13039\/100001279","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001279","name":"Orthopaedic Research and Education Foundation","doi-asserted-by":"publisher","award":["TAF\u201021\u2010059"],"award-info":[{"award-number":["TAF\u201021\u2010059"]}],"id":[{"id":"10.13039\/100001279","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chronic conditions associated with aging have proven difficult to prevent or treat. Senescence is a cell fate defined by loss of proliferative capacity and the development of a pro\u2010inflammatory senescence\u2010associated secretory phenotype comprised of cytokines\/chemokines, proteases, and other factors that promotes age\u2010related diseases. Specifically, an increase in senescent peripheral blood mononuclear cells (PBMCs), including T cells, is associated with conditions like frailty, rheumatoid arthritis, and bone loss. However, it is unknown if the percentage of senescent PBMCs associated with age\u2010associated orthopedic decline could be used for potential diagnostic or prognostic use in orthopedics. Here, we report senescent cell detection using the fluorescent compound C\n                    <jats:sub>12<\/jats:sub>\n                    FDG to quantify PBMCs senescence across a large cohort of healthy and osteoarthritic patients. There is an increase in the percent of circulating C\n                    <jats:sub>12<\/jats:sub>\n                    FDG\n                    <jats:sup>+<\/jats:sup>\n                    PBMCs that is commensurate with increases in age and senescence\u2010related serum biomarkers. Interestingly, C\n                    <jats:sub>12<\/jats:sub>\n                    FDG\n                    <jats:sup>+<\/jats:sup>\n                    PBMCs and T cells also were found to be elevated in patients with mild to moderate osteoarthritis, a progressive joint disease that is strongly associated with inflammation. The percent of C\n                    <jats:sub>12<\/jats:sub>\n                    FDG\n                    <jats:sup>+<\/jats:sup>\n                    PBMCs and age\u2010related serum biomarkers were decreased in a small subgroup of study participants taking the senolytic drug fisetin. These results demonstrate quantifiable measurements in a large group of participants that could create a composite score of healthy aging sensitive enough to detect changes following senolytic therapy and may predict age\u2010related orthopedic decline. Detection of peripheral senescence in PBMCs and subsets using C\n                    <jats:sub>12<\/jats:sub>\n                    FDG may be clinically useful for quantifying cellular senescence and determining how and if it plays a pathological role in osteoarthritic progression.\n                  <\/jats:p>","DOI":"10.1111\/acel.14113","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T06:04:01Z","timestamp":1714975441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Clinical validation of\n                    <scp>\n                      C\n                      <sub>12<\/sub>\n                      FDG\n                    <\/scp>\n                    as a marker associated with senescence and osteoarthritic phenotypes"],"prefix":"10.1111","volume":"23","author":[{"given":"William S.","family":"Hambright","sequence":"first","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6949-1862","authenticated-orcid":false,"given":"Victoria R.","family":"Duke","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam D.","family":"Goff","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex W.","family":"Goff","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas T.","family":"Minas","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3087-9661","authenticated-orcid":false,"given":"Heidi","family":"Kloser","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Huard","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Guo","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5529-6599","authenticated-orcid":false,"given":"Aiping","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mullen","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Su","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Tchkonia","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0387-5059","authenticated-orcid":false,"given":"Jair M.","family":"Espindola\u00a0Netto","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1068-7099","authenticated-orcid":false,"given":"Paul D.","family":"Robbins","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Biophysics, Institute on the Biology of Aging and Metabolism University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1074-1385","authenticated-orcid":false,"given":"Laura J.","family":"Niedernhofer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology and Biophysics, Institute on the Biology of Aging and Metabolism University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Kirkland","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic  Rochester Minnesota USA"},{"name":"Division of General Internal Medicine, Department of Medicine Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9808-8888","authenticated-orcid":false,"given":"Chelsea S.","family":"Bahney","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"},{"name":"Orthopaedic Trauma Institute University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0358-3331","authenticated-orcid":false,"given":"Marc","family":"Philippon","sequence":"additional","affiliation":[{"name":"Center for Regenerative Sports Medicine Steadman Philippon Research Institute  Vail Colorado USA"},{"name":"The Steadman Clinic  Vail Colorado 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Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.14113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T08:19:02Z","timestamp":1716452342000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.14113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/acel.14113"],"URL":"https:\/\/doi.org\/10.1111\/acel.14113","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5]]},"assertion":[{"value":"2023-05-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14113"}}