{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:43:37Z","timestamp":1790765017189,"version":"4.1.0"},"reference-count":75,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T00:00:00Z","timestamp":1709510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["23\u2010BR\u201002\u201003"],"award-info":[{"award-number":["23\u2010BR\u201002\u201003"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["23\u2010BR\u201004\u201002"],"award-info":[{"award-number":["23\u2010BR\u201004\u201002"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["23\u2010BR\u201002\u201013"],"award-info":[{"award-number":["23\u2010BR\u201002\u201013"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["23\u2010BR\u201005\u201001"],"award-info":[{"award-number":["23\u2010BR\u201005\u201001"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["22\u2010CoE\u2010BT\u201002"],"award-info":[{"award-number":["22\u2010CoE\u2010BT\u201002"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["A0801043001"],"award-info":[{"award-number":["A0801043001"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An early diagnosis of Alzheimer's disease is crucial as treatment efficacy is limited to the early stages. However, the current diagnostic methods are limited to mid or later stages of disease development owing to the limitations of clinical examinations and amyloid plaque imaging. Therefore, this study aimed to identify molecular signatures including blood plasma extracellular vesicle biomarker proteins associated with Alzheimer's disease to aid early\u2010stage diagnosis. The hippocampus, cortex, and blood plasma extracellular vesicles of 3\u2010 and 6\u2010month\u2010old 5xFAD mice were analyzed using quantitative proteomics. Subsequent bioinformatics and biochemical analyses were performed to compare the molecular signatures between wild type and 5xFAD mice across different brain regions and age groups to elucidate disease pathology. There was a unique signature of significantly altered proteins in the hippocampal and cortical proteomes of 3\u2010 and 6\u2010month\u2010old mice. The plasma extracellular vesicle proteomes exhibited distinct informatic features compared with the other proteomes. Furthermore, the regulation of several canonical pathways (including phosphatidylinositol 3\u2010kinase\/protein kinase B signaling) differed between the hippocampus and cortex. Twelve potential biomarkers for the detection of early\u2010stage Alzheimer's disease were identified and validated using plasma extracellular vesicles from stage\u2010divided patients. Finally, integrin \u03b1\u2010IIb, creatine kinase M\u2010type, filamin C, glutamine \u03b3\u2010glutamyltransferase 2, and lysosomal \u03b1\u2010mannosidase were selected as distinguishing biomarkers for healthy individuals and early\u2010stage Alzheimer's disease patients using machine learning modeling with approximately 79% accuracy. Our study identified novel early\u2010stage molecular signatures associated with the progression of Alzheimer's disease, thereby providing novel insights into its pathogenesis.<\/jats:p>","DOI":"10.1111\/acel.14137","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T14:03:27Z","timestamp":1709561007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Multi\u2010proteomic analyses of 5xFAD mice reveal new molecular signatures of early\u2010stage Alzheimer's disease"],"prefix":"10.1111","volume":"23","author":[{"given":"Seulah","family":"Lee","sequence":"first","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuk\u2010In","family":"Jang","sequence":"additional","affiliation":[{"name":"Cognitive Science Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagyeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon Suk","family":"Jo","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"},{"name":"Department of Brain\u2010Cognitive Science Daegu\u2010Gyeongbuk Institute of Science and Technology (DGIST)  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayoung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geuna","family":"Park","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungwon","family":"Bae","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang Woo","family":"Kwon","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Hyeok","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Brain\u2010Cognitive Science Daegu\u2010Gyeongbuk Institute of Science and Technology (DGIST)  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Seok","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Brain\u2010Cognitive Science Daegu\u2010Gyeongbuk Institute of Science and Technology (DGIST)  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chany","family":"Lee","sequence":"additional","affiliation":[{"name":"Cognitive Science Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4090-3832","authenticated-orcid":false,"given":"Jong Hyuk","family":"Yoon","sequence":"additional","affiliation":[{"name":"Neurodegenerative Diseases Research Group Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0891988703258321"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010020\u201002013\u20101"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010017\u20100283\u20105"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.10.003"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20107704\u20108_17"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336918.2016.1236899"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2012.11.024"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.20517\/2347\u20108659.2017.50"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0mo00186d"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19092603"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052761"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0757\u201012.2012"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00276"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2014.08.028"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.11.006"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/trc2.12295"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.11.094"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyv106"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010160567"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2119804119"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010170045"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159x13666150716165726"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C500244200"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014\u2010015\u20109091\u20108"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300677110"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000111342"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010019\u20100709\u20106"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2016.00177"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853899908995889"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010017\u20100231\u20107"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2534\u201016.2017"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010022\u201000665\u20102"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900218"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116079"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0531\u20105565(00)00113\u20103"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1461145711000459"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010020\u20100593\u20101"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/171464"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.842973"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181ec173d"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2021.06.006"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.56"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.3233\/ADR\u2010170049"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248\u2010018\u20100211\u2010z"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207885"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.06.017"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1202\u201006.2006"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dadm.2016.06.003"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2016.09.014"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201001150\u2010x"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086641"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.2147\/DNND.S228939"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2013.08.001"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010019\u20100133\u20103"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.14283\/jpad.2018.34"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.23.2845"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001353\u20108"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbas.2023.100078"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.565479"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/cn500099f"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12522"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106183"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv262"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582\u2010022\u201000749\u2010z"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2016.206"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172504199"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00039"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/psyg.12685"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010017\u20100294\u20102"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.4103\/0366\u20106999.186646"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.14506.1"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dadm.2016.04.001"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750\u20101326\u20107\u20108"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1145\/1081870.1081911"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S264498"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.14137","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T19:46:06Z","timestamp":1718135166000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.14137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,4]]},"references-count":75,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/acel.14137"],"URL":"https:\/\/doi.org\/10.1111\/acel.14137","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,4]]},"assertion":[{"value":"2023-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14137"}}