{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:58:01Z","timestamp":1790013481509,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1201404"],"award-info":[{"award-number":["2021YFA1201404"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuromuscular junction (NMJ) degeneration is one of pathological factors of sarcopenia. Low\u2010magnitude high\u2010frequency vibration (LMHFV) was reported effective in alleviating the sarcopenia progress. However, no previous study has investigated treatment effects of LMHFV targeting NMJ degeneration in sarcopenia. We first compared morphological differences of NMJ between sarcopenic and non\u2010sarcopenic subjects, as well as young and old C57BL\/6 mice. We then systematically characterized the age\u2010related degeneration of NMJ in SAMP8 against its control strain, SAMR1 mice, from 3 to 12\u2009months old. We also investigated effects of LMHFV in SAMP8 on the maintenance of NMJ during the onset of sarcopenia with respect to the Agrin\u2010LRP4\u2010MuSK\u2010Dok7 pathway and investigated the mechanism related to ERK1\/2 signaling. We observed sarcopenic\/old NMJ presented increased acetylcholine receptors (AChRs) cluster fragmentation and discontinuity than non\u2010sarcopenic\/young NMJ. In SAMP8, NMJ degeneration (morphologically at 6\u2009months and functionally at 8\u2009months) was observed associated with the sarcopenia onset (10\u2009months). SAMR1 showed improved NMJ morphology and function compared with SAMP8 at 10\u2009months. Skeletal muscle performance was improved at Month 4 post\u2010LMHFV treatment. Vibration group presented improved NMJ function at Months 2 and 6 posttreatment, accompanied with alleviated morphological degeneration at Month 4 posttreatment. LMHFV increased Dok7 expression at Month 4 posttreatment. In\u00a0vitro, LMHFV could promote AChRs clustering in myotubes by increasing Dok7 expression through suppressing ERK1\/2 phosphorylation. In conclusion, NMJ degeneration was observed associated with the sarcopenia onset in SAMP8. LMHFV may attenuate NMJ degeneration and sarcopenia progression by increasing Dok7 expression through suppressing ERK1\/2 phosphorylation.<\/jats:p>","DOI":"10.1111\/acel.14156","type":"journal-article","created":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T07:26:48Z","timestamp":1711524408000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prevention of age\u2010related neuromuscular junction degeneration in sarcopenia by low\u2010magnitude high\u2010frequency vibration"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhengyuan","family":"Bao","sequence":"first","affiliation":[{"name":"Musculoskleletal Research Laboratory, Department of Orthopaedics and Traumatology, Prince of Wales Hospital The Chinese University of Hong Kong  Hong Kong SAR China"},{"name":"Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9328-4521","authenticated-orcid":false,"given":"Can","family":"Cui","sequence":"additional","affiliation":[{"name":"Musculoskleletal Research Laboratory, Department of Orthopaedics and Traumatology, Prince of Wales Hospital The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoran","family":"Liu","sequence":"additional","affiliation":[{"name":"Musculoskleletal Research Laboratory, Department of Orthopaedics and Traumatology, Prince of Wales Hospital The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Long","sequence":"additional","affiliation":[{"name":"Musculoskleletal Research Laboratory, Department of Orthopaedics and Traumatology, Prince of Wales Hospital The 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