{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:21:31Z","timestamp":1791177691352,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T00:00:00Z","timestamp":1718928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY033915"],"award-info":[{"award-number":["R01EY033915"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA046934"],"award-info":[{"award-number":["P30CA046934"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Posterior capsule opacification (PCO) is a common complication after cataract surgery. Residual lens epithelial cells (LECs) on the anterior lens capsule, after cataract surgery, migrate to the posterior lens capsule and undergo transdifferentiation into myofibroblast\u2010like cells. Those cells synthesize excessive amounts of extracellular matrix and contribute to fibrosis during PCO. Cellular senescence, a phenomenon that increases with aging, has been implicated in several fibrotic diseases. Here, we have investigated the prevalence of senescent LECs within the lens posterior capsule and the ability of advanced glycation end products (AGEs) in lens capsules to induce senescence, contributing to PCO. Aged lens capsules from pseudophakic human cadaver eyes showed the presence of senescent LECs. In human capsular bags, LECs showed an age\u2010dependent increase in senescence after 28\u2009days of culture. Human LECs cultured on aged lens capsules for 3\u2009days underwent senescence; this effect was not seen in LECs cultured on young lens capsules. Human LECs cultured on an AGE\u2010modified extracellular matrix (ECM\u2010AGEs) showed an AGE\u2010concentration\u2010dependent increase in the expression of senescence markers and reactive oxygen species (ROS) levels. Treatment with a RAGE antagonist and ROS inhibitor reduced the expression of senescence and fibrotic markers. Additionally, conditioned media from ECM\u2010AGEs\u2010treated cells induced the expression of fibrotic markers in na\u00efve LECs. Together, these suggest that AGEs in the capsule induce senescence of LECs, which triggers the mesenchymal transition of neighboring non\u2010senescent LECs and contributes to PCO.<\/jats:p>","DOI":"10.1111\/acel.14249","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T06:39:35Z","timestamp":1719038375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Lens capsule advanced glycation end products induce senescence in epithelial cells: Implications for secondary cataracts"],"prefix":"10.1111","volume":"23","author":[{"given":"Grace","family":"Cooksley","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Hyun","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rooban B.","family":"Nahomi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Rankenberg","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J. 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