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Here we show that rat anti\u2010HGF monoclonal antibody (mAb) 1H41C10, which was raised in\u2010house against a synthetic peptide FTSNPEVR<jats:sub>nitro<\/jats:sub>Y<jats:sub>198<\/jats:sub>EV, a site well\u2010conserved in mammals, functions to confer resistance to nitration dysfunction on HGF. 1H41C10 was characterized by recognizing both nitrated and non\u2010nitrated HGF with different affinities as revealed by Western blotting, indicating that the paratope of 1H41C10 may bind to the immediate vicinity of Y198. Subsequent experiments showed that 1H41C10\u2010bound HGF resists peroxynitrite\u2010induced nitration of Y198. A companion mAb\u20101H42F4 presented similar immuno\u2010reactivity, but did not protect Y198 nitration, and thus served as the control. Importantly, 1H41C10\u2010HGF also withstood Y250 nitration to retain c\u2010met binding and satellite cell activation functions in culture. The Fab region of 1H41C10 exerts resistivity to Y250 nitration possibly due to its localization in the immediate vicinity to Y250, as supported by an additional set of experiments showing that the 1H41C10\u2010Fab confers Y250\u2010nitration resistance which the Fc segment does not. Findings highlight the in\u00a0vitro preventive impact of 1H41C10 on HGF nitration\u2010dysfunction that strongly impairs myogenic stem cell dynamics, potentially pioneering cogent strategies for counteracting or treating age\u2010related muscle atrophy with fibrosis (including sarcopenia and frailty) and the therapeutic application of investigational HGF drugs.<\/jats:p>","DOI":"10.1111\/acel.14337","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T09:31:00Z","timestamp":1726738260000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vitro immuno\u2010prevention of nitration\/dysfunction of myogenic stem cell activator <scp>HGF<\/scp>, towards developing a strategy for age\u2010related muscle atrophy"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7592-2863","authenticated-orcid":false,"given":"Sakiho","family":"Tanaka","sequence":"first","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Graduate School of Agriculture, Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4457-0809","authenticated-orcid":false,"given":"Alaa","family":"Elgaabari","sequence":"additional","affiliation":[{"name":"Department of Animal and Marine Bioresource Sciences Graduate School of Agriculture, Kyushu University  Fukuoka Japan"},{"name":"Department of Physiology, Faculty of Veterinary Medicine Kafrelsheikh University  Kafrelsheikh 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