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As\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants are propagated, their fertility deteriorates prior to the onset of a reproductive arrest phenotype that resembles a starvation\u2010induced stress response. We found that late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants with substantially reduced fertility were long\u2010lived, whereas early\u2010 or mid\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants had normal lifespans. Loss of the stress response transcription factor DAF\u201016 caused mid\u2010 or late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants to live very short lives, whereas overexpression of DAF\u201016 enabled both mid\u2010 and late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants to live long. Cytoplasmic P\u2010bodies that respond to stress increased in long\u2010lived late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants and were transmitted to F1 but not F2 cross\u2010progeny. Moreover, moderate levels of heritable stress shorten late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutant longevity when DAF\u201016 or P bodies are deficient. Together, these results suggest that the longevity of late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants is a hormetic stress response. However, dauer larvae that occur in response to stress were not observed in late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants. Small germ cell nucleoli that depended on germline DAF\u201016 were present in late\u2010generation\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutants but were not necessary for their longevity. We propose that\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    mutant germ cells transmit a form of heritable stress, high levels of which promote longevity and strongly reduce fertility. The heritable stress transmitted by\n                    <jats:italic>prg\u20101<\/jats:italic>\n                    \/Piwi mutant germ cells may be generally relevant to epigenetic inheritance of longevity.\n                  <\/jats:p>","DOI":"10.1111\/acel.14350","type":"journal-article","created":{"date-parts":[[2024,11,9]],"date-time":"2024-11-09T04:14:11Z","timestamp":1731125651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Piwi mutant germ cells transmit a form of heritable stress that promotes longevity"],"prefix":"10.1111","volume":"24","author":[{"given":"Bree","family":"Heestand","sequence":"first","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Lineberger Comprehensive Cancer Center University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan H.","family":"Lister\u2010Shimauchi","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Frenk","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6365-2194","authenticated-orcid":false,"given":"Shawn","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Genetics University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Department of Biology University of North Carolina  Chapel Hill North Carolina USA"},{"name":"Lineberger Comprehensive Cancer Center University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.17912\/micropub.biology.000210"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178343"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-020-0462-7"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.120.303565"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.01.039"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.170.1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53757"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-015-9601-0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2010.10.005"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.200920"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.07.010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.13.1578"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.148"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10572"},{"key":"e_1_2_10_16_1","unstructured":"Heestand B. 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