{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:47:07Z","timestamp":1791474427968,"version":"4.3.4"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1300604"],"award-info":[{"award-number":["2021YFA1300604"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI144997"],"award-info":[{"award-number":["AI144997"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["MH118164"],"award-info":[{"award-number":["MH118164"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["82025024"],"award-info":[{"award-number":["82025024"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000026","name":"National Institute on Drug Abuse","doi-asserted-by":"publisher","award":["DA047807"],"award-info":[{"award-number":["DA047807"]}],"id":[{"id":"10.13039\/100000026","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"Common Fund","doi-asserted-by":"publisher","award":["CA241694"],"award-info":[{"award-number":["CA241694"]}],"id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302637"],"award-info":[{"award-number":["82302637"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Extracellular vesicles (EVs) are nanosized particles that are released by various cell types and play vital roles in intercellular communication. They carry biological molecules reflecting the physiological and pathological states of their source cells and tissues, showing potential as biomarkers. However, the impact of demographic factors like age and sex on the properties of blood plasma EVs remains underexplored. This study aims to fill this gap by evaluating how these factors influence the particle count and proteomic profiles of plasma EV preparations and corresponding protein fractions. Plasma samples from 120 healthy volunteers were collected and pooled into six groups: young males (age: 27.6\u2009\u00b1\u20094.0), young females (27.4\u2009\u00b1\u20093.8), middle\u2010aged males (48.8\u2009\u00b1\u20093.8), middle\u2010aged females (48.9\u2009\u00b1\u20093.9), old males (69.3\u2009\u00b1\u20093.9), and old females (69.4\u2009\u00b1\u20094.3). EV\u2010 and protein\u2010enriched fractions were separated by size\u2010exclusion chromatography (SEC). Fractions were characterized for particle number concentration and protein composition to identify characteristics affected by age and biological sex. Plasma EVs and corresponding protein fractions exhibited distinct characteristics, with differential enrichment of markers related to EVs and other blood components, including lipoproteins. Proteomic profiles of both EVs and protein fractions displayed sex\u2010 and age\u2010dependent differences. Differentially abundant proteins displayed functions previously identified in the context of aging and sex differences, highlighting their utility as biomarkers. Age and sex significantly affect the characteristics of plasma EVs and proteins, potentially influencing their efficacy and interpretation as biomarkers in clinical applications. This study lays the groundwork for detailed mechanistic research to understand how EVs mediate age\u2010 and sex\u2010related effects in health.<\/jats:p>","DOI":"10.1111\/acel.14356","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T09:39:24Z","timestamp":1728293964000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Associations of age and sex with characteristics of extracellular vesicles and protein\u2010enriched fractions of blood plasma"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1749-963X","authenticated-orcid":false,"given":"Yiyao","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"},{"name":"Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiannan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Urology Renji Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanting","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W.","family":"Witwer","sequence":"additional","affiliation":[{"name":"Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine  Baltimore Maryland USA"},{"name":"Department of Neurology Johns Hopkins University School of Medicine  Baltimore Maryland USA"},{"name":"Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102517"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12328"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010021\u201003796\u20109"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw419"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2016.07.012"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v3.23430"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2021.101410"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12202"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b00631"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1007"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1647027"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2017.189"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12044"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201001386\u2010z"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2017.04.019"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12093"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13293\u2010019\u20100246\u20106"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glx105"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574\u2010018\u20100059\u20104"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33641"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13293\u2010015\u20100028\u20108"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0278368"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/inmd.20230016"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010220322"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/qad.0000000000003487"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.15386\/cjmed\u2010880"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1555410"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.09.019"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13283"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/7854_2022_309"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010023\u201001741\u2010x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2021.0527"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27031"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2013.05.009"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.72676"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201053640\u20101"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.1059"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006802\u20101"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2016\u20101137"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2023.111792"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010030\u201012298\u20100_3"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.68"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010022\u201000196\u2010x"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311767"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24316"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6742792"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.86394"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010030\u201025650\u20100_4"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12140"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101488"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4185"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12122"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI158448"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.56035"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010019\u20106089\u2010z"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12207"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.06.020"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002\u2010021604"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12154"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12979\u2010019\u20100172\u20109"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.14356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T19:23:08Z","timestamp":1744312988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.14356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,7]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/acel.14356"],"URL":"https:\/\/doi.org\/10.1111\/acel.14356","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,7]]},"assertion":[{"value":"2024-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14356"}}