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Senescent cells are individual cells that are irreversibly cell cycle arrested and can accumulate across the human lifespan due to cell\u2010intrinsic and \u2010extrinsic stressors. With an atlas of single\u2010cell RNA\u2010sequencing and spatial transcriptomics, epidermal and dermal senescence and its effects were investigated, with a focus on melanocytes and fibroblasts. Photoaging due to ultraviolet light exposure was associated with higher burdens of senescent cells, a sign of biological aging, compared to chronological aging. A skin\u2010specific cellular senescence gene set, termed SenSkin\u2122, was curated and confirmed to be elevated in the context of photoaging, chronological aging, and non\u2010replicating\n                    <jats:italic>CDKN1A<\/jats:italic>\n                    + (p21) cells. In the epidermis, senescent melanocytes were associated with elevated melanin synthesis, suggesting haphazard pigmentation, while in the dermis, senescent reticular dermal fibroblasts were associated with decreased collagen and elastic fiber synthesis. Spatial analysis revealed the tendency for senescent cells to cluster, particularly in photoaged skin. This work proposes a strategy for characterizing age\u2010related skin dysfunction through the lens of cellular senescence and suggests a role for senescent epidermal cells (i.e., melanocytes) and senescent dermal cells (i.e., reticular dermal fibroblasts) in age\u2010related skin sequelae.\n                  <\/jats:p>","DOI":"10.1111\/acel.14358","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T01:32:00Z","timestamp":1728264720000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Mapping epidermal and dermal cellular senescence in human skin aging"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7161-0787","authenticated-orcid":false,"given":"Grace T.","family":"Yu","sequence":"first","affiliation":[{"name":"Mayo Clinic Medical Scientist Training Program Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic Alix School of Medicine  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarisse","family":"Ganier","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapy and Regenerative Medicine King's College London, Guy's Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Allison","sequence":"additional","affiliation":[{"name":"Indiana University School of Public Health  Bloomington Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Tchkonia","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine Center for Gerotherapeutics, Cedars\u2010Sinai Medical Center  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2936-4372","authenticated-orcid":false,"given":"Sundeep","family":"Khosla","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Department of Medicine Mayo Clinic  Rochester Minnesota USA"},{"name":"Robert and Arlene Kogod Center on Aging Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Kirkland","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine Center for Gerotherapeutics, Cedars\u2010Sinai Medical Center  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus D.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapy and Regenerative Medicine King's College London, Guy's Hospital  London UK"},{"name":"St. John's Institute of Dermatology King's College London, Guy's Hospital  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saranya P.","family":"Wyles","sequence":"additional","affiliation":[{"name":"Robert and Arlene Kogod Center on Aging Mayo Clinic  Rochester Minnesota USA"},{"name":"Department of Dermatology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,6]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.797747"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2023.03.1679"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2021.01.008"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13602"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502856102"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14995"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae456"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2023.05.031"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2313326120"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010023\u201000446\u20106"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10123111"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2070"},{"key":"e_1_2_12_15_1","doi-asserted-by":"crossref","unstructured":"Jupe S.(2012).Elastic fibre formation. 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