{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:46:14Z","timestamp":1790361974663,"version":"4.1.0"},"reference-count":100,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ultraviolet B (UVB) radiation is a major contributor to skin photoaging. Although mainly absorbed by the epidermis, UVB photons managing to penetrate the upper dermis affect human dermal fibroblasts (HDFs), leading, among others, to the accumulation of senescent cells. In\u00a0vitro studies have shown that repeated exposures to subcytotoxic UVB radiation doses provoke HDFs' premature senescence shortly after the end of the treatment period. Here, we found that repetitive exposures to non\u2010cytotoxic UVB radiation doses after several days lead to mixed cultures, containing both senescent cells and fibroblasts resisting senescence. \u201cResistant\u201d fibroblasts were more resilient to a novel intense UVB radiation stimulus. RNA\u2010seq analysis revealed that ERCC6, encoding Cockayne syndrome group B (CSB) protein, is up\u2010regulated in resistant HDFs compared to young and senescent cells. CSB was found to be a key molecule conferring protection toward UVB\u2010induced cytotoxicity and senescence, as siRNA\u2010mediated CSB loss\u2010of\u2010expression rendered HDFs significantly more susceptible to a high UVB radiation dose, while cells from a CSB\u2010deficient patient were found to be more sensitive to UVB\u2010mediated toxicity, as well as senescence. UVB\u2010resistant HDFs remained normal (able to undergo replicative senescence) and non\u2010tumorigenic. Even though they formed a distinct population in\u2010between young and senescent cells, resistant HDFs retained numerous tissue\u2010impairing characteristics of the senescence\u2010associated secretory phenotype, including increased matrix metalloprotease activity and promotion of epidermoid tumor xenografts in immunodeficient mice. Collectively, here we describe a novel subpopulation of HDFs showing increased resistance to UVB\u2010mediated premature senescence while retaining undesirable traits that may negatively affect skin homeostasis.<\/jats:p>","DOI":"10.1111\/acel.14422","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T06:43:14Z","timestamp":1734590594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A subset of human dermal fibroblasts overexpressing Cockayne syndrome group B protein resist UVB radiation\u2010mediated premature senescence"],"prefix":"10.1111","volume":"24","author":[{"given":"Asimina","family":"Fotopoulou","sequence":"first","affiliation":[{"name":"Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications National Centre for Scientific Research \u201cDemokritos\u201d  Athens Greece"},{"name":"Department of Chemistry University of Patras  Patras Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria T.","family":"Angelopoulou","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications National Centre for Scientific Research \u201cDemokritos\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7550-1975","authenticated-orcid":false,"given":"Harris","family":"Pratsinis","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications National Centre for Scientific Research \u201cDemokritos\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Mavrogonatou","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications National Centre for Scientific Research \u201cDemokritos\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9828-3963","authenticated-orcid":false,"given":"Dimitris","family":"Kletsas","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications National Centre for Scientific Research \u201cDemokritos\u201d  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1990.tb08452.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.04.004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2012.02.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203372"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201490041"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409147200"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1404.002"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2007.10.014"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox4020248"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-605X\/aae7ba"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5327.831"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12020444"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11102011"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12368"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2012.01200.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jidsymp.1998.2"},{"issue":"5","key":"e_1_2_10_19_1","first-page":"632","article-title":"UVB\u2010induced senescence of human dermal fibroblasts involves impairment of proteasome and enhanced Autophagic activity","volume":"72","author":"Cavinato M.","year":"2017","journal-title":"The Journals of Gerontology. 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