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Using human single\u2010cell RNA sequencing and laser capture microdissection, we identified C\u2010X\u2010C Motif Chemokine Ligand 13 (CXCL13) as a key chemokine secreted by senescent prostate epithelial cells. CXCL13 recruits CD4<jats:sup>+<\/jats:sup> T cells via the C\u2010X\u2010C Chemokine Receptor Type 5 (CXCR5) receptor, facilitating immune recognition through human leukocyte antigen\u2013DR isotype (HLA\u2010DR) and promoting senescent cell clearance. Functional assays revealed that CD4<jats:sup>+<\/jats:sup> cytotoxic T lymphocytes (CTLs) mediate this clearance, while regulatory T cells (Tregs) suppress it, forming a functional dichotomy. Immunohistochemistry, transwell migration, and co\u2010culture assays confirmed this CXCL13\u2013CXCR5\u2013HLA\u2010DR axis. In a testosterone\u2010induced BPH mouse model, CXCL13 treatment enhanced CD4<jats:sup>+<\/jats:sup> T cell infiltration and reduced epithelial senescence, while CD4<jats:sup>+<\/jats:sup> T cell depletion reversed these effects. Single\u2010cell transcriptomics in mice further validated increased CXCL13 expression and CD4<jats:sup>+<\/jats:sup> T cell engagement. These findings uncover a critical immune surveillance mechanism in BPH and suggest that targeting the CXCL13\u2013CD4<jats:sup>+<\/jats:sup> T cell axis may offer a novel therapeutic strategy for age\u2010related prostate enlargement.<\/jats:p>","DOI":"10.1111\/acel.70180","type":"journal-article","created":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T16:09:55Z","timestamp":1753632595000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Single\u2010Cell Sequencing Reveals That <scp>CD4<\/scp><sup>+<\/sup> T Cells Eliminate Senescent Prostate Epithelium to Delay Progression of Benign Prostatic Hyperplasia"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0584-1808","authenticated-orcid":false,"given":"Zheng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, Beijing Key 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