{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:45:58Z","timestamp":1792017958468,"version":"4.4.0"},"reference-count":103,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P30642\u2010b28"],"award-info":[{"award-number":["P30642\u2010b28"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P34191"],"award-info":[{"award-number":["P34191"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The process of aging is associated with a decline in cell, tissue, and organ function, leading to a range of health problems. Increasing evidence indicates that dietary restriction can counteract age\u2010dependent effects and improve health and longevity in whole organisms, but less is known about the influence of aging and the impact of nutrition on individual organs of an organism. In this study, we examined the intestine of the very short\u2010lived aging model system, the African turquoise killifish (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Nothobranchius furzeri<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), throughout its lifetime. We investigated the effects of age and nutrition on the preservation of gut tissue at stages corresponding to human neonatal, adolescent, adult, and old age, and integrated morphological measurements, histology, and transcriptomics. The intestinal mucosa is characterized by folds and intervening interfold regions, where intestinal stem cells localize. The stem cells occur in clusters, and the cycle time of stem cells increases with age. We also observed a reduction in intestinal length and volume with age. Age\u2010dependent transcriptomic profiling revealed significant changes in the expression of peripheral circadian clock genes and stem cell niche markers. Notably, the majority of these genes maintained their adult gene expression levels in old age following intermittent fasting during adulthood. Therefore, our results demonstrate that the decline in structural intestinal tissue homeostasis is associated with a decline in stem cell activity that can be counteracted by intermittent fasting. Since the intestinal mucosa of killifish is similar to that of mammals, the results of this study can be translated to general gut biology.\n                  <\/jats:p>","DOI":"10.1111\/acel.70229","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T06:00:40Z","timestamp":1758607240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Aging Reduces Intestinal Stem Cell Activity in Killifish and Intermittent Fasting Reverses Intestinal Gene Expression Patterns"],"prefix":"10.1111","volume":"24","author":[{"given":"Michael","family":"Kothmayer","sequence":"first","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Laffer","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Widmayer","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2630-4809","authenticated-orcid":false,"given":"Elmar E.","family":"Ebner","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"},{"name":"Unit of Laboratory Animal Pathology, Center of Pathobiology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7704-2140","authenticated-orcid":false,"given":"Fehima","family":"Ugarak","sequence":"additional","affiliation":[{"name":"Division of Anatomy, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"},{"name":"Institut FEMTO\u2010ST Universit\u00e9 de Franche\u2010Comt\u00e9, CNRS  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Martin","sequence":"additional","affiliation":[{"name":"Unit of Laboratory Animal Pathology, Center of Pathobiology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan H.","family":"Geyer","sequence":"additional","affiliation":[{"name":"Division of Anatomy, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kareem","family":"Elsayad","sequence":"additional","affiliation":[{"name":"Division of Anatomy, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang J.","family":"Weninger","sequence":"additional","affiliation":[{"name":"Division of Anatomy, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Lagger","sequence":"additional","affiliation":[{"name":"Unit of Laboratory Animal Pathology, Center of Pathobiology University of Veterinary Medicine Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara","family":"Weipoltshammer","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Pusch","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9570-5692","authenticated-orcid":false,"given":"Christian","family":"Sch\u00f6fer","sequence":"additional","affiliation":[{"name":"Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201022922\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.134098"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1097\u20102765(02)00542\u20107"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2018.08.010"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742\u20104658.2008.06480.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06196"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203788"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.182675"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12257"},{"key":"e_1_2_10_11_1","volume-title":"Dynamic Light Scattering, With Applications to Chemistry, Biology, and Physics","author":"Berne B. J.","year":"1977"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001159"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2022.2054636"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010023\u201003845\u20108"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.2669"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001410407"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.06.004"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13963"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.11.019"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adk3079"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.25225\/jvb.21074"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1088\/978-0-7503-3059-6ch39"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10570\u2010020\u201003075\u2010z"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.020"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107565"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010018\u20100081\u2010y"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3791\/56071"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.09.001"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010021\u201000411\u20104"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101212\u20105"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735\u2010018\u20109761\u20108"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1049"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.14149"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2014.898326"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/iob\/obac003"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00219868"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01076482"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2019.05.001"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529209095998"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.175018"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101905"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.16"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2002054"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010018\u20104570\u20108"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104957"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199750"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.04.005"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.10.015"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi2671"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2020.01.008"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.02.012"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69008"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.11.008"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2023.152116"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.04.001"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.08.011"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9010103"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00493829"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.12.011"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.092971cm"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-96998-5_25"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2022.02.003"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02347181"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045942\u20104"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.08.001"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2012.10.001"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101383\u20100"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2016.06.006"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2022.01.011"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00219230"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2016.05.002"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2024.3977"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.041"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2009.06.020"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.4161\/nucl.36229"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.03.024"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00401226"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.150"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427\u2010012\u20100422\u20108"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517694039"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.104280"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474\u20109726.2009.00455.x"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2006.06.039"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.03.007"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms16083"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.12.038"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12134"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2004.10.009"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2021.02.001"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201011\u2010392"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587\u2010022\u201000357\u2010y"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg1371"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110311"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111982"},{"key":"e_1_2_10_102_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915\u2010023\u201001667\u20102"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11163"},{"key":"e_1_2_10_104_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.627143"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/acel.70229","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/acel.70229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T21:59:31Z","timestamp":1771279171000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/acel.70229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,22]]},"references-count":103,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/acel.70229"],"URL":"https:\/\/doi.org\/10.1111\/acel.70229","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,22]]},"assertion":[{"value":"2025-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70229"}}